LOCUS       sequence-130343-         609 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transcriptional unit for (human codon optimized) Cas9 plant 
            expression driven by the 35S promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 609)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pEGB2alpha2 35s:hCas9:tNos (GB0639)
FEATURES             Location/Qualifiers
     source          1..609
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tgaagacgaa agggcctcga tgtgacgcct agtttttata ggattgatgt cntgatagga
       61 atggtttctt agacgtcaga gttggnactt ttcggggana tgtgcgcgga anccctattt
      121 gtgtatttnt ctaaatacat tcaaatatgt atccgctcat gnganaataa ccctgataaa
      181 tgcttcaatg aatgggaccg actcgcgcnt gagacgaagc ttgacgtaan cctaagagag
      241 aagagcgttt attataataa tcggatattt aaaaggnngt gannaggttt atccgttcgt
      301 ccatttgnat gtgcatgcca acnacagggt tcccctcggg atcaangtgn tttgatccag
      361 cccctccgct gctatggtgn agncggcttc tgacgttcgg tgcagccgtc atctgaaaac
      421 gacatgncnc acaaggtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg
      481 ntttcttgtc gcgtgtttta gtcncataaa gtagantact tgcgactgga accggagaca
      541 ttacgccatg aacaagagcg ccgccnctgg cctgctgggc tatgcccgcg tcancaccga
      601 cgancagga
//
