LOCUS       sequence-130348-         703 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone containing BTK-PH with no stop codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Ariotti N, Hall TE, Rae J, Ferguson C, McMahon KA, Martel N, Webb 
            RE, Webb RI, Teasdale RD, Parton RG
  TITLE     Modular Detection of GFP-Labeled Proteins for Rapid Screening by 
            Electron Microscopy in Cells and Organisms.
  JOURNAL   Dev Cell. 2015 Nov 23;35(4):513-25. doi: 
            10.1016/j.devcel.2015.10.016. Epub 2015 Nov 12.
  PUBMED    26585296
REFERENCE   2  (bases 1 to 703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2015.10.016"; journalName: "Dev Cell"; date: 
            "2015-11-23- 23"; volume: "35"; issue: "4"; pages: "513-25"
COMMENT     Sequence Label: pME-BTK-PH
FEATURES             Location/Qualifiers
     source          1..703
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    59..83
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      84..93
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    complement(622..646)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ttgactgata gtgacctgtt cgttgcaaca aattgatgag caatgctttt ttataatgcc
       61 aactttgtac aaaaaagcag gctgccacca tggccgcagt gattctggag agcatctttc
      121 tgaagcgatc ccaacagaaa aagaaaacat cacctctaaa cttcaagaag cgcctgtttc
      181 tcttgaccgt gcacaaactc tcctactatg agtatgactt tgaacgtggg agaagaggca
      241 gtaagaaggg ttcaatagat gttgagaaga tcacttgtgt tgaaacagtg gttcctgaaa
      301 aaaatcctcc tccagaaaga cagattccga gaagaggtga agagtccagt gaaatggagc
      361 aaatttcaat cattgaaagg ttcccttatc ccttccaggt tgtatatgat gaagggcctc
      421 tctacgtctt ctccccaact gaagaactaa ggaagcggtg gattcaccag ctcaaaaacg
      481 taatccggta caacagtgat ctggttcaga aatatcaccc ttgcttctgg atcgatgggc
      541 agtatctctg ctgctctcag acagccaaaa atgctatggg ctgccaaatt ttggagaaca
      601 ggaatggaag cttaaaaccg gacccagctt tcttgtacaa agttggcatt ataagaaagc
      661 attgcttatc aatttgttgc aacgaacagg tcactatcag tca
//
