LOCUS       sequence-130350-         751 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transcriptional unit for human codon optimized with mutated (D10A, 
            H840A) and inactivated catalytic domains Cas9 protein plant 
            expression driven by the 35S promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 751)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 751)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pEGB 35S:dCas9:Tnos (GB1191)
FEATURES             Location/Qualifiers
     source          1..751
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggagaggact ccggtatttt tacaacaatt accacaacaa aacaaacaac aaacaacatt
       61 acaatttact attctagtcg aaatggacaa gaagtactcc attgggctcg ctatcggcac
      121 aaacagcgtc ggctgggccg tcattacgga cgagtacaag gtgccgagca aaaaattcaa
      181 agttctgggc aataccgatc gccacagcat aaagaagaac ctcattggcg ccctcctgtt
      241 cgactccggg gaaacggccg aagccacgcg gctcaaaaga acagcacggc gcagatatac
      301 ccgcagaaag aatcggatct gctacctgca ggagatcttt agtaatgaga tggctaaggt
      361 ggatgactct ttcttccata ggctggagga gtcctttttg gtggaggagg ataaaaagca
      421 cgagcgccac ccaatctttg gcaatatcgt ggacgaggtg gcgtaccatg aaaagtaccc
      481 aaccatatat catctgagga agaagcttgt agacagtact gataaggctg acttgcggtt
      541 gatctatctc gcgctggcgc atatgatcaa atttcgggga cacttcctca tcgaggggga
      601 cctgaaccca gacaacagcg atgtcgacaa actctttatc caactggttc agacttacaa
      661 tcagcttttc gaagagaacc cgatcaacgc atccggagtt gacgccaaag caatcctgag
      721 cgctaggctg tccaaatccc ggcggctcga a
//
