LOCUS       sequence-130353-         604 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transcriptional unit for human codon optimized with mutated (D10A, 
            H840A) and inactivated catalytic domains Cas9 protein plant 
            expression driven by the 35S promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 604)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 604)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pEGB 35S:dCas9:Tnos (GB1191)
FEATURES             Location/Qualifiers
     source          1..604
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(70..81)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 tggcgttcag cagctgccgc caataatttt tcattttctt gacaacttct tctgagggga
       61 cgttatcact cttccctcta tttttatcgg atcttgtcaa cactttatta tcaatagaat
      121 catctttgag aaaagactgg ggcacgatgg catccacgtc gtagtcggag agccgattga
      181 tgtccagttc ctgatccacg tacatgtccc tgccgttctg caggtagtac aggtagagct
      241 tctcattctg aagctgggtg ttttcaactg ggtgttcctt aaggatttgg gaccccagtt
      301 cttttatacc ctcttcaatc ctcttcatcc tttccctact gttcttctgt cccttctggg
      361 tagtttggtt ctctcgggcc atctcgataa cgatattctc gggcttatgc cttcccatta
      421 ctttgacgag ttcatccacg accttaacgg tctgcagtat tccctttttg atagctgggc
      481 tacctgcaag attagcgatg tgctcgtgga gactgtcccc ctggccagaa acttgtgctt
      541 tctggatgtc ctccttaaag gtgagagagt catcatggat caactgcatg aagttccggt
      601 tggc
//
