LOCUS       sequence-130355-         724 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transcriptional unit for human codon optimized with mutated (D10A, 
            H840A) and inactivated catalytic domains Cas9 protein plant 
            expression driven by the 35S promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 724)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 724)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pEGB 35S:dCas9:Tnos (GB1191)
FEATURES             Location/Qualifiers
     source          1..724
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(38..56)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    240..264
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
ORIGIN
        1 atcgggaatt gccaagctaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta
       61 taggttaatg tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat
      121 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      181 agacaataac cctgataaat gcttcaataa tgggaccgac tcgcgcttga gacgaagctt
      241 gacaggatat attggcgggt aaacctaaga gaaaagagcg tttattagaa taatcggata
      301 tttaaaaggg cgtgaaaagg tttatccgtt cgtccatttg tatgtgcatg ccaaccacag
      361 ggttcccctc gggatcaaag tactttgatc caacccctcc gctgctatag tgcagtcggc
      421 ttctgacgtt cagtgcagcc gtcatctgaa aacgacatgt cgcacaagtc ctaagttacg
      481 cgacaggctg ccgccctgcc cttttcctgg cgttttcttg tcgcgtgttt tagtcgcata
      541 aagtagaata cttgcgacta gaaccggaga cattacgcca tgaacaagag cgccgccgct
      601 ggcctgctgg gctatgcccg cgtcagcacc gacgaccagg acttgaccaa ccaacgggcc
      661 gaactgcacg cggccggctg caccaagctg ttttccgaga agatcaccgg caccaggcgc
      721 gacc
//
