LOCUS       sequence-130356-         727 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Transcriptional unit for human codon optimized with mutated (D10A, 
            H840A) and inactivated catalytic domains Cas9 protein plant 
            expression driven by the 35S promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 727)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 727)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pEGB 35S:dCas9:Tnos (GB1191)
FEATURES             Location/Qualifiers
     source          1..727
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    475..499
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
ORIGIN
        1 tcgcggcctt gagcaagacg tttcccgttg aatatggctc ataacagaac ttattatttc
       61 cttcctcttt tctacagtat ttaaagatac cccaagaagc taattataac aagacgaact
      121 ccaattcact gttccttgca ttctaaaacc ttaaatacca gaaaacagct ttttcaaagt
      181 tgttttcaaa gttggcgtat aacatagtat cgacggagcc gattttgaaa ccgcggtgat
      241 cacaggcagc aacgctctgt catcgttaca atcaacatgc taccctccgc gagatcatcc
      301 gtgtttcaaa cccggcagct tagttgccgt tcttccgaat agcatcggta acatgagcaa
      361 agtctgccgc cttacaacgg ctctcccgct gacgccgtcc cggactgatg ggctgcctgt
      421 atcgagtggt gattttgtgc cgagctgccg gtcggggagc tgttggctgg ctggtggcag
      481 gatatattgt ggtgtaaaca taacaagctt cgtctcagtc aggagactag agccaagctg
      541 atctcctttg ccccggagat caccatggac gactttctct atctctacga tctaggaaga
      601 aagttcgacg gagaaggtga cgataccatg ttcaccaccg ataatgagaa gattagcctc
      661 ttcaatttca gaaagaatgc tgacccacag atggttagag aggcctacgc ggcaggtctg
      721 atcaaga
//
