LOCUS       sequence-130357-         817 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Twister plasmid to swap inserts from an alpha2 or alpha2R vector to 
            any omega level vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 817)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pDGB1alpha1_SF (GB0106)
FEATURES             Location/Qualifiers
     source          1..817
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    151..175
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     misc_feature    363..385
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
ORIGIN
        1 cccctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
       61 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
      121 agatctcaaa caaacacata cagcgactta gtttacccgc caatatatcc tgtcagaatt
      181 cgtctcatga cagcgaaatg attgatgaag aacaatggtg gatgaagaac aaagaaggag
      241 ggagcttttg ttcaagatga acaaagaaca atagtggatg aagaacaaag tgaaaaaaat
      301 aaaaaaaaat gtatggttaa ataaagagta aagttaccat tgagactcct gagacgaatt
      361 cgttacacca caatatatcc tgccaagatc taattccggg gatcggaaat ccagaagccc
      421 gagaggttgc cgcctttcgg gctttttctt tttcaaaaaa aaaaatttat aaaacgatct
      481 gttgcggccg gccgccgggt tgtgggcaaa ggcgctcgac ggtgggcaac cgcttgcggt
      541 tgtccacggg cggagccggt gcgcgtagcg cattgtccac aagccaaggg cgaccaataa
      601 ttgatatata tattcataat tgaaaagcta attgaacata ctacttgctg taactacttg
      661 ccggagcgag gggtgtttgc aagctgttga tctgaaaggg ctattagcgt tctcacgtgc
      721 ctttttgatt agcgatttca cgtgacctta ttagcgattt cacgtactcc gattagcgat
      781 ttcacgtacc ctgattagcg atttcacgtg gatagtt
//
