LOCUS       sequence-130361-        1095 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  specific sgRNA against mouse Dicer1 gene cloned in the pX330 
            backbone (Addgene Number 42230). Generation of Dicer1 knockout 
            mESCs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Bodak M, Cirera-Salinas D, Yu J, Ngondo RP, Ciaudo C
  TITLE     Dicer, a new regulator of pluripotency exit and LINE-1 elements in 
            mouse embryonic stem cells.
  JOURNAL   FEBS Open Bio. 2017 Jan 11;7(2):204-220. doi: 
            10.1002/2211-5463.12174. eCollection 2017 Feb.
  PUBMED    28174687
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/2211-5463.12174"; journalName: "FEBS Open Bio"; date: 
            "2017-01-11- 11"; volume: "7"; issue: "2"; pages: "204-220"
COMMENT     Sequence Label: pX330-sgRNA_Dicer_1
FEATURES             Location/Qualifiers
     source          1..1095
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(11..58)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ggaattctta ctttttcttt tttgcctggc cggccttttt cgtggccgcc ggccttttgt
       61 cgcctcccag ctgagacagg tcgatccgtg tctcgtacag gccggtgatg ctctggtgga
      121 tcagggtggc gtccagcacc tctttggtgc tggtgtacct cttccggtcg atggtggtgt
      181 caaagtactt gaaggcggca ggggctccca gattggtcag ggtaaacagg tggatgatat
      241 tctcggcctg ctctctgatg ggcttatccc ggtgcttgtt gtaggcggac agcactttgt
      301 ccagattagc gtcggccagg atcactctct tggagaactc gctgatctgc tcgatgatct
      361 cgtccaggta gtgcttgtgc tgttccacaa acagctgttt ctgctcatta tcctcggggg
      421 agcccttcag cttctcatag tggctggcca ggtacaggaa gttcacatat ttggagggca
      481 gggccagttc gtttcccttc tgcagttcgc cggcagaggc cagcattctc ttccggccgt
      541 tttccagctc gaacagggag tacttaggca gcttgatgat caggtccttt ttcacttctt
      601 tgtagccctt ggcttccaga aagtcgatgg gattcttctc gaagctgctt ctttccatga
      661 tggtgatccc cagcagctct ttcacactct tcagtttctt ggacttgccc ttttccactt
      721 tggccaccac cagcacagaa taggccacgg tggggctgtc gaagccgccg tacttcttag
      781 ggtcccagtc cttctttctg gcgatcagct tatcgctgtt cctcttgggc aggatagact
      841 ctttgctgaa gccgcctgtc tgcacctcgg tctttttcac gatattcact tggggcatgc
      901 tcagcacttt ccgcacggtg gcaaaatccc ggcccttatc ccacacgatc tccccggttt
      961 cgccgtttgt ctcgatcaga ggccgcttcc ggatctcgcc gttggccagg gtaatctcgg
     1021 tcttgaaaaa gttcatgatg ttgctgtaga agaagtactt ggcggtagcc ttgccgattt
     1081 cctgctcgct cttgg
//
