LOCUS       sequence-130362-        1022 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  specific sgRNA against mouse Dicer1 gene cloned in the pX330 
            backbone (Addgene Number 42230). Generation of Dicer1 knockout 
            mESCs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1022)
  AUTHORS   Bodak M, Cirera-Salinas D, Yu J, Ngondo RP, Ciaudo C
  TITLE     Dicer, a new regulator of pluripotency exit and LINE-1 elements in 
            mouse embryonic stem cells.
  JOURNAL   FEBS Open Bio. 2017 Jan 11;7(2):204-220. doi: 
            10.1002/2211-5463.12174. eCollection 2017 Feb.
  PUBMED    28174687
REFERENCE   2  (bases 1 to 1022)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/2211-5463.12174"; journalName: "FEBS Open Bio"; date: 
            "2017-01-11- 11"; volume: "7"; issue: "2"; pages: "204-220"
COMMENT     Sequence Label: pX330-sgRNA_Dicer_1
FEATURES             Location/Qualifiers
     source          1..1022
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cagttcgctc aggccgcctc tctcggcctt ggtcagattg tcgaactttc tctgggtaat
       61 cagcttggcg ttcagcagct gccgccagta gttcttcatc ttcttcacga cctcttcgga
      121 gggcacgttg tcgctcttgc cccggttctt gtcgcttctg gtcagcacct tgttgtcgat
      181 ggagtcgtcc ttcagaaagc tctgaggcac gatatggtcc acatcgtagt cggacagccg
      241 gttgatgtcc agttcctggt ccacgtacat atcccgccca ttctgcaggt agtacaggta
      301 cagcttctcg ttctgcagct gggtgttttc cacggggtgt tctttcagga tctggctgcc
      361 cagctctttg atgccctctt cgatccgctt cattctctcg cggctgttct tctgtccctt
      421 ctgggtggtc tggttctctc tggccatttc gatcacgatg ttctcgggct tgtgccggcc
      481 catcactttc acgagctcgt ccaccacctt cactgtctgc aggatgccct tcttaatggc
      541 ggggctgccg gccagattgg caatgtgctc gtgcaggcta tcgccctggc cggacacctg
      601 ggctttctgg atgtcctctt taaaggtcag gctgtcgtcg tggatcagct gcatgaagtt
      661 tctgttggcg aagccgtcgg acttcaggaa anccaggatt gtcttgccgg actgcttgtc
      721 ccggatgccg ttgatcagct tccggctcag cctgccccag ccggtgtatc tccgccgctt
      781 cagctgcttc atcactttgt cgtcgaacag gtgggcatag gttttcagcc gttcctcgat
      841 catctctctg tcctcaaaca gtgtcagggt cagcacgata tcttccagaa tgtcctcgtt
      901 ttcctcattg tccaggaagt ccttgtcctt gataattttc agcagatcgt ggtatgtgcc
      961 cagggaggcg ttgaaccgat cttccacgcc ggagatttcc acggagtcga agcactcgat
     1021 tt
//
