LOCUS       sequence-130363-         918 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  specific sgRNA against mouse Dicer1 gene cloned in the pX330 
            backbone (Addgene Number 42230). Generation of Dicer1 knockout 
            mESCs..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 918)
  AUTHORS   Bodak M, Cirera-Salinas D, Yu J, Ngondo RP, Ciaudo C
  TITLE     Dicer, a new regulator of pluripotency exit and LINE-1 elements in 
            mouse embryonic stem cells.
  JOURNAL   FEBS Open Bio. 2017 Jan 11;7(2):204-220. doi: 
            10.1002/2211-5463.12174. eCollection 2017 Feb.
  PUBMED    28174687
REFERENCE   2  (bases 1 to 918)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/2211-5463.12174"; journalName: "FEBS Open Bio"; date: 
            "2017-01-11- 11"; volume: "7"; issue: "2"; pages: "204-220"
COMMENT     Sequence Label: pX330-sgRNA_Dicer_1
FEATURES             Location/Qualifiers
     source          1..918
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      46..55
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             55..120
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     CDS             127..147
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 cctggagcac ctgcctgaaa tcactttttt tcaggttgga ccggtgccac catggactat
       61 aaggaccacg acggagacta caaggatcat gatattgatt acaaagacga tgacgataag
      121 atggccccaa agaagaagcg gaaggtcggt atccacggag tcccagcagc cgacaagaag
      181 tacagcatcg gcctggacat cggcaccaac tctgtgggct gggccgtgat caccgacgag
      241 tacaaggtgc ccagcaagaa attcaaggtg ctgggcaaca ccgaccggca cagcatcaag
      301 aagaacctga tcggagccct gctgttcgac agcggcgaaa cagccgaggc cacccggctg
      361 aagagaaccg ccagaagaag atacaccaga cggaagaacc ggatctgcta tctgcaagag
      421 atcttcagca acgagatggc caaggtggac gacagcttct tccacagact ggaagagtcc
      481 ttcctggtgg aagaggataa gaagcacgag cggcacccca tcttcggcaa catcgtggac
      541 gaggtggcct accacgagaa gtaccccacc atctaccacc tgagaaagaa actggtggac
      601 agcaccgaca aggccgacct gcggctgatc tatctggccc tggcccacat gatcaagttc
      661 cggggccact tcctgatcga gggcgacctg aaccccgaca acagcgacgt ggacaagctg
      721 ttcatccagc tggtgcagac ctacaaccag ctgttcgagg aaaaccccat caacgccagc
      781 ggcgtggacg ccaaggccat cctgtctgcc agactgagca agagcagacg gctggaaaat
      841 ctgatcgccc agctgcccgg cgagaagaag aatggcctgt tcggaaacct gattgccctg
      901 agcctgggcc tgaccccc
//
