LOCUS       sequence-130365-         622 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  specific sgRNA against mouse Dicer1 gene cloned in the pX330 
            backbone (Addgene Number 42230). Generation of.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 622)
  AUTHORS   Bodak M, Cirera-Salinas D, Yu J, Ngondo RP, Ciaudo C
  TITLE     Dicer, a new regulator of pluripotency exit and LINE-1 elements in 
            mouse embryonic stem cells.
  JOURNAL   FEBS Open Bio. 2017 Jan 11;7(2):204-220. doi: 
            10.1002/2211-5463.12174. eCollection 2017 Feb.
  PUBMED    28174687
REFERENCE   2  (bases 1 to 622)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1002/2211-5463.12174"; journalName: "FEBS Open Bio"; date: 
            "2017-01-11- 11"; volume: "7"; issue: "2"; pages: "204-220"
COMMENT     Sequence Label: pX330-sgRNA_Dicer_2
FEATURES             Location/Qualifiers
     source          1..622
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(328..348)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(355..420)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     regulatory      420..429
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ttccagtctg tggaagaagc tgtcgtccac cttggccatc tcgttgctga agatctcttg
       61 cagatagcag atccggttct tccgtctggt gtatcttctt ctggcggttc tcttcagccg
      121 ggtggcctcg gctgtttcgc cgctgtcgaa cagcagggct ccgatcaggt tcttcttgat
      181 gctgtgccgg tcggtgttgc ccagcacctt gaatttcttg ctgggcacct tgtactcgtc
      241 ggtgatcacg gcccagccca cagagttggt gccgatgtcc aggccgatgc tgtacttctt
      301 gtcggctgct gggactccgt ggataccgac cttccgcttc ttctttgggg ccatcttatc
      361 gtcatcgtct ttgtaatcaa tatcatgatc cttgtagtct ccgtcgtggt ccttatagtc
      421 catggtggca ccggtccaac ctgaaaaaaa gtgatttcag gcaggtgctc caggtaatta
      481 aacattaata ccccaccaac caaccatccc ttaaaccctt acctcttgct cagctaatta
      541 cagcccggag gagaagggcc gtcccgcccg ctcacctgtg ggagtaacgc ggtcagtcag
      601 agccggggcg ggcggcgcga gg
//
