LOCUS       sequence-130366-        1086 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  This vector can be used for homologous recombination and allows to 
            obtain recombinant neomycin resistant fluorescent cells that can 
            loose fluorescence after Cre expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1086)
  AUTHORS   Gian Luca Forni
  TITLE     The plasmid is a modification of the p076 pPNT6 with the green 
            fluorescent protein gene cloned within the LoxP sites in xbaI site 
            next to Neomycin cassette
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1086)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pPNT6-eGFP
FEATURES             Location/Qualifiers
     source          1..1086
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    49..97
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     122..141
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
ORIGIN
        1 gtggggtatc gacagagtgc cagccctggg accgaacccc gcgtttatga acaaacgacc
       61 caacacccgt gcgttttatt ctgtcttttt attgccgtca tagcgcgggt tccttccggt
      121 attgtctcct tccgtgtttc agttagcctc ccccatctcc cgggcaaacg tgcgcgccag
      181 gtcgcagatc gtcggtatgg agccgggggt ggtgacgtgg gtctggacca tcccggaggt
      241 aagttgcagc agggcgtccc ggcagccggc gggcgattgg tcgtaatcca ggataaagac
      301 gtgcatggga cggaggcgtt tggccaagac gtccaaggcc caggcaaaca cgttgtacag
      361 gtcgccgttg ggggccagca actcgggggc ccgaaacagg gtaaataacg tgtccccgat
      421 atggggtcgt gggcccgcgt tgctctgggg ctcggcaccc tggggcggca cggccgtccc
      481 cgaaagctgt ccccaatcct cccgccacga cccgccgccc tgcagatacc gcaccgtatt
      541 ggcaagcagc ccgtaaacgc ggcgaatcgc ggccagcata gccaggtcaa gccgctcgcc
      601 ggggcgctgg cgtttggcca ggcggtcgat gtgtctgtcc tccggaaggg cccccaacac
      661 gatgtttgtg ccgggcaagg tcggcgggat gagggccacg aacgccagca cggcctgggg
      721 ggtcatgctg cccataaggt atcgcgcggc cgggtagcac aggagggcgg cgatgggatg
      781 gcggtcgaag atgagggtga gggccggggg cggggcatgt gagctcccag cctccccccc
      841 gatatgagga gccagaacgg cgtcggtcac ggcataaggc atgcccattg ttatctgggc
      901 gcttgtcatt accaccgccg cgtccccggc cgatatctca ccctggtcga ggcggtgttg
      961 tgtggtgtag atgttcgcga ttgtctcgga agcccccagc acctgccagt aagtcatcgg
     1021 ctcgggtacg tagacgatat cgtcgcgcga acccagggcc accagcagtt gcgtggtggt
     1081 ggtttt
//
