LOCUS       sequence-130387-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector driving AKT-APEX2 (Human AKT-PH domain, 
            [marks PI(3,4)P2 and PI(3,4,5)P3] fused to EM peroxidase marker). 
            Also contains bicistronially expressed cytoplasmic red reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Ariotti N, Hall TE, Rae J, Ferguson C, McMahon KA, Martel N, Webb 
            RE, Webb RI, Teasdale RD, Parton RG
  TITLE     Modular Detection of GFP-Labeled Proteins for Rapid Screening by 
            Electron Microscopy in Cells and Organisms.
  JOURNAL   Dev Cell. 2015 Nov 23;35(4):513-25. doi: 
            10.1016/j.devcel.2015.10.016. Epub 2015 Nov 12.
  PUBMED    26585296
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2015.10.016"; journalName: "Dev Cell"; date: 
            "2015-11-23- 23"; volume: "35"; issue: "4"; pages: "513-25"
COMMENT     Sequence Label: AKT-APEX2-P2A-mKate2
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    34..54
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             complement(60..755)
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="VSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKA
                     VEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMALK
                     LVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHR"
     CDS             complement(774..830)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 atacgactca ctatagttct agaggctcga gtgcaactat gtataataaa gttgactagt
       61 catctgtgcc ccagtttgct agggaggtcg cagtatctgg ccacagccac ctcgtgctgc
      121 tcgacgtagg tctctttgtc ggcctccttg attctttcca gtcttctgtc cacatagtag
      181 acgccgggca tcttgaggtt cttagcgggt ttcttggatc tgtatgtggt cttcaagttg
      241 cagatcaggt ggcccccgcc cacgagcttc agggccatgt cggctctgcc ttccaggccg
      301 ccgtcagcgg ggtacagggt ctcggtggag gcctcccagc cgagtgtttt cttctgcatc
      361 acagggccgt tggatgggaa gttcacccct ctgatcttga cgttgtagat gaggcagccg
      421 tcctggaggc tggtgtcctg ggtagcggtc agcacgcccc cgtcttcgta tgtggtgact
      481 ctctcccatg tgaagccctc ggggaaggac tgcttaaaga agtcggggat gccctgggtg
      541 tggttgatga aggttttgct gccgtacatg aagctggtag ccaggatgtc gaaggcgaag
      601 gggagagggc cgccctcgac cgccttgatt ctcatggtct gggtgccctc gtagggcttg
      661 ccttcgccct cggatgtgca cttgaagtgg tggttgttca cggtgccctc catgtacagc
      721 ttcatgtgca tgttctcctt aatcagctcg ctcacggtac cgcttccagg agcaggaccg
      781 gggttttctt ccacgtctcc tgcttgcttt aacagagaga agttcgtggc tccggatccg
      841 gcatcagcaa
//
