LOCUS       sequence-13039-e        3612 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3612)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3612)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1145-HQ858689
FEATURES             Location/Qualifiers
     source          1..3612
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1169..1268)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1285..1304)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1286..1304)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1309..1325)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1309..1325)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1427..1446)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1438..2247
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1505..1524)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2394..2982
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2883..2902
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3136..3153
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3312..3339)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3431..3517)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3566..3588
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3580..3597
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3581..3597
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atggccgccg gagccggagc cggagatgaa gagaaagatg acgcggtgct ccctgggttc
      181 cggttccacc cgaccgacga ggagctcgtc acgttctacc tccgccggaa ggtggccagg
      241 aagccgctca gcatggagat catcaaggag atggacatct acaagcatga tccatgggac
      301 ctccctaagg cgagcacggc tgctggtggc gaaaaggagt ggtacttctt ctgcctgaga
      361 ggaaggaagt accggaacag catcaggccc aacagggtca ccggctccgg cttctggaag
      421 gccaccggca tcgaccggca tatctaccct gccaccgcga accccgtcga gtccgtgtcc
      481 gtcgggctca agaagtccct cgtgtactac cgcggcagcg ccggcaaggg caccaagacc
      541 gactggatga tgcacgagtt ccgcctcccg ccggccgccg cctcgccctc gccaacgccg
      601 acgagcatgc aagaagctga ggtgtggacc atctgcagga tcttcaagag gaacatcgcc
      661 tacaagaggc agccgcagct gccggcgtgg aggcagcagc agcagtccag ctccaacacg
      721 gggagcttcg agtccgacgg cggcggcggc ggggacgagt acatgaaccg ctgcctgccg
      781 gtgccggcca cggccacggc caccgctcat cagatcggcg gcatgctgaa cggaggaggc
      841 gtcgtcagtg ccactggcag cagcagcagc agtttcttca gggacaggga gagcgtgcac
      901 ggcggccagc aattccactt ccacgagcag tggctgagcc gcttccccgc cgcgccagca
      961 gtcgagcaga agccgcagct cctggacccg tcggcgatgg ccatcgcgtt ccatcagaac
     1021 gacgaccaga gcgtcgccac cgctgccgct gccgctgcga gcgagtgctg caaggacggg
     1081 tactactggg acgagatcgc gaggttcatg gaggtcaacg atccaacggt gcttcacgac
     1141 tgtagatacg ctaagggtgg gcgcgccgac ccagctttct tgtacaaagt tggcattata
     1201 agaaagcatt gcttatcaat ttgttgcaac gaacaggtca ctatcagtca aaataaaatc
     1261 attatttgcc atccagctga tatcccctat agtgagtcgt attacatggt catagctgtt
     1321 tcctggcagc tctggcccgt gtctcaaaat ctctgatgtt acattgcaca agataaaaat
     1381 atatcatcat gaacaataaa actgtctgct tacataaaca gtaatacaag gggtgttatg
     1441 agccatattc aacgggaaac gtcgaggccg cgattaaatt ccaacatgga tgctgattta
     1501 tatgggtata aatgggctcg cgataatgtc gggcaatcag gtgcgacaat ctatcgcttg
     1561 tatgggaagc ccgatgcgcc agagttgttt ctgaaacatg gcaaaggtag cgttgccaat
     1621 gatgttacag atgagatggt cagactaaac tggctgacgg aatttatgcc tcttccgacc
     1681 atcaagcatt ttatccgtac tcctgatgat gcatggttac tcaccactgc gatccccgga
     1741 aaaacagcat tccaggtatt agaagaatat cctgattcag gtgaaaatat tgttgatgcg
     1801 ctggcagtgt tcctgcgccg gttgcattcg attcctgttt gtaattgtcc ttttaacagc
     1861 gatcgcgtat ttcgtctcgc tcaggcgcaa tcacgaatga ataacggttt ggttgatgcg
     1921 agtgattttg atgacgagcg taatggctgg cctgttgaac aagtctggaa agaaatgcat
     1981 aaacttttgc cattctcacc ggattcagtc gtcactcatg gtgatttctc acttgataac
     2041 cttatttttg acgaggggaa attaataggt tgtattgatg ttggacgagt cggaatcgca
     2101 gaccgatacc aggatcttgc catcctatgg aactgcctcg gtgagttttc tccttcatta
     2161 cagaaacggc tttttcaaaa atatggtatt gataatcctg atatgaataa attgcagttt
     2221 catttgatgc tcgatgagtt tttctaatca gaattggtta attggttgta acactggcag
     2281 agcattacgc tgacttgacg ggacggcgca agctcatgac caaaatccct taacgtgagt
     2341 tacgcgtcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
     2401 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
     2461 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
     2521 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
     2581 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
     2641 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
     2701 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
     2761 gaactgagat acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag
     2821 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
     2881 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
     2941 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
     3001 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
     3061 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
     3121 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
     3181 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
     3241 tggaaagcgg gcagtgagcg caacgcaatt aatacgcgta ccgctagcca ggaagagttt
     3301 gtagaaacgc aaaaaggcca tccgtcagga tggccttctg cttagtttga tgcctggcag
     3361 tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg cttcacaacg ttcaaatccg
     3421 ctcccggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa
     3481 aggcccagtc ttccgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc
     3541 gcgttaacgc tagcatggat gttttcccag tcacgacgtt gtaaaacgac ggccagtctt
     3601 aagctcgggc cc
//
