LOCUS       sequence-130392-         852 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector driving APEX2-TAPP1 (Human TAPP1-PH 
            domain, [marks PI(3,4)P2] fused to EM peroxidase marker). Also 
            contains bicistronially expressed cytoplasmic red reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 852)
  AUTHORS   Ariotti N, Hall TE, Rae J, Ferguson C, McMahon KA, Martel N, Webb 
            RE, Webb RI, Teasdale RD, Parton RG
  TITLE     Modular Detection of GFP-Labeled Proteins for Rapid Screening by 
            Electron Microscopy in Cells and Organisms.
  JOURNAL   Dev Cell. 2015 Nov 23;35(4):513-25. doi: 
            10.1016/j.devcel.2015.10.016. Epub 2015 Nov 12.
  PUBMED    26585296
REFERENCE   2  (bases 1 to 852)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2015.10.016"; journalName: "Dev Cell"; date: 
            "2015-11-23- 23"; volume: "35"; issue: "4"; pages: "513-25"
COMMENT     Sequence Label: mKate2-P2A-APEX2-TAPP1-PH
FEATURES             Location/Qualifiers
     source          1..852
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    31..51
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    422..446
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cgactcacta tagttctaga ggctcgagtg caactatgta taataaagtt gtcagggatg
       61 ctcagaagac gcagatctgc cgggaccccg ctgtgctaca atggcgccag agactgcttt
      121 aatccaactg tgcatctctt cagggctatc agcctgcaca tagaaagttc gagacgttgt
      181 tacaatttca aagaggttgt ccctcatcat tatgtcgctt tgcttacatt cctggacttt
      241 atgaacctct ttaagtggta ttacgcgaag aggttccttt tccagttcag atttgaagta
      301 gcctattgtg ttttcatcca attgaaaata tcttctcttc cagtttttca tcactgctcc
      361 ttgttttaca caatatccag ctttgataac cgcactatct tgaggtggtt taggagtaaa
      421 caccactttg tacaagaaag ctgggtcgac ggcatcagca aacccaagct cggaaagctt
      481 ttggtgagcc tcagcgtaat cagcaaagaa ggcatcttcg tccgctgcat atttgtcaac
      541 gagagggcgg aatacagggt cagacaaaag agccttgtca gaaggtagct gaaggagacc
      601 ttccttctca ccactcaaca actccgtgaa gtatgagttg tcgaaaataa gaggattaga
      661 ggtccaggga ccctcaaatc cagaacgctc cttgtgtgca gctccaatag tgtgaccccc
      721 agatagagca acgatatctt ggtcagtaag ccccatagct ttgccaaaca catctctcaa
      781 atggtcagaa cccttagtgg gatcgggcaa gcgaccctct ggtggtggct caggcttgtc
      841 ctctcttcca gg
//
