LOCUS       sequence-130405-         974 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentivirus expressing mTagBFP2 with UTR sensor cassette and 
            hygromycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 974)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, 
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 974)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-18"; volume: "6:16836. doi"; pages: " 
            10.1038/srep16836"
COMMENT     Sequence Label: pGK:HygroR-CMV:mTagBFP2-A_UTR
FEATURES             Location/Qualifiers
     source          1..974
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        213..592
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        593..796
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     747..767
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             884..901
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 caatcgtccg atccggagcc gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg
       61 ccgtctggac cgatggctgt gtagaagtac tcgccgatag tggaaaccga cgccccagca
      121 ctcgtccgag ggcaaaggaa tagaaaccgc tgttcctagg aatccgaggc cttttgcgct
      181 gcttcgcgat gtacgggcca gatatacgcg ttgacattga ttattgacta gttattaata
      241 gtaatcaatt acggggtcat tagttcatag cccatatatg gagttccgcg ttacataact
      301 tacggtaaat ggcccgcctg actgaccgcc caacgacccc cgcccattga cgtcaataat
      361 gacgtatgtt cccatagtaa cgccaatagg gactttccat tgacgtcaat gggtggagta
      421 tttacggtaa actgcccact tggcagtaca tcaagtgtat catatgccaa gtacgccccc
      481 tattgacgtc aatgactgta aatggcccgc ctggcattat gcccagtaca tgaccttatg
      541 ggactttcct acttggcagt acatctacgt attagtcatc gctattacca tggtgatgcg
      601 gttttggcag tacatcaatg ggcgtggata gcggtttgac tcacggggat ttccaagtct
      661 ccaccccatt gacgtcaatg ggagtttgtt ttggcaccaa aatcaacggg actttccaaa
      721 atgtcgtaac aactccgccc cattgacgca aatgggcggt aggcgtgtac ggtgggaggt
      781 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
      841 tgacccgaca ttagcgctac agcttaagcg gaccatgggg agccaccatc accatcacca
      901 tggcagatct atgagcgagc tgattaagga gaacatgcac atgaagctgt acatggaggg
      961 caccgtggac aacc
//
