LOCUS       sequence-130406-         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentivirus expressing mTagBFP2 with UTR sensor cassette and 
            hygromycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Akama-Garren EH, Joshi NS, Tammela T, Chang GP, Wagner BL, Lee DY, 
            Rideout Iii WM, Papagiannakopoulos T, Xue W, Jacks T
  TITLE     A Modular Assembly Platform for Rapid Generation of DNA Constructs.
  JOURNAL   Sci Rep. 2016 Feb 18;6:16836. doi: 10.1038/srep16836.
  PUBMED    26887506
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-18"; volume: "6:16836. doi"; pages: " 
            10.1038/srep16836"
COMMENT     Sequence Label: pGK:HygroR-CMV:mTagBFP2-A_UTR
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(159..860)
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
                     VEGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
                     LVGGSHLIANAKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
                     DLPSKLGHKLN"
     CDS             complement(870..887)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 acatagttaa gaataccagt caatctttca caaattttgt aatccagagg ttgattatcg
       61 ggcgcgccgc ggtctagaag agtaattcaa ccccaaacaa caacgtttcc atgcaaggac
      121 tattacacat gtacttgtag tggatggtgg tatactcatt aattaagctt gtgccccagt
      181 ttgctaggga ggtcgcagta tctggccact gccacctcgt gctgctcgac gtaggtctcg
      241 ttgttggcct ccttgattct ttccagtctg tagtccacat agtagacgcc aggcatcttg
      301 aggttcttag cgggtttctt ggatctatat gtggtcttgg cgtttgcgat cagatggctc
      361 ccgcccacga gcttcagggc catgtcgttt ctgccttcca ggccgccgtc agcggggtac
      421 agcgtctcgg tgaaggcctc ccagccgagt gttttcttct gcatcacagg gccgttggat
      481 gtgaagttca cccctctgat cttgacgttg tagatgaggc agccgtcctg gaggctggtg
      541 tcctgggtag cggtcagcac gcccccgtct tcgtatgtgg tgactctctc ccatgtgaag
      601 ccctcaggga aggactgctt gaagaagtcg gggatgccct gggtgtggtt gatgaaggtc
      661 ttgctgccgt agaggaagct agtagccagg atgtcgaagg cgaaggggag agggccgccc
      721 tcgaccacct tgattctcat ggtctgggtg ccctcgtagg gcttgccttc gccctcggat
      781 gtgcacttga agtgatggtt gtccacggtg ccctccatgt acagcttcat gtgcatgttc
      841 tccttaatca gctcgctcat agatctgcca tggtgatggt gatggtggct ccccatggtc
      901 cgcttaagct gtagcgctaa tgtcggg
//
