LOCUS       sequence-130414-         975 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a biosensor comprised of a fast-maturing pH-stable red fluorescent 
            protein fused to a pH-sensitive green fluorescent protein variant. 
            Cytosolic localization..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 975)
  AUTHORS   Rosado CJ, Mijaljica D, Hatzinisiriou I, Prescott M, Devenish RJ
  TITLE     Rosella: a fluorescent pH-biosensor for reporting vacuolar turnover 
            of cytosol and organelles in yeast.
  JOURNAL   Autophagy. 2008 Feb;4(2):205-13. Epub 2007 Nov 21.
  PUBMED    18094608
REFERENCE   2  (bases 1 to 975)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Autophagy."; date: "2008-02"; volume: "4(2)"; pages: "205-13. Epub 
            2007 Nov 21"
COMMENT     Sequence Label: pAS1NB c Rosella I
FEATURES             Location/Qualifiers
     source          1..975
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    201..253
                     /label=MCS
                     /note="multiple cloning site"
     regulatory      263..272
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             269..943
                     /codon_start=1
                     /product="rapidly maturing tetrameric variant of DsRed 
                     fluorescent protein (Bevis and Glick, 2002)"
                     /label=DsRed-Express
                     /note="mammalian codon-optimized"
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKVYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGRFIYKVKFIGVNFPSDGPVMQKKTMGWEPSTERLYPRDGVLKGEIHK
                     ALKLKDGGHYLVEFKSIYMAKKPVQLPGYYYVDSKLDITSHNEDYTIVEQYERTEGRHH
                     LFL"
     primer_bind     complement(399..417)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     complement(449..469)
                     /label=DsRed1-N
                     /note="DsRed1, reverse primer"
     primer_bind     667..686
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     861..884
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gggtggttta gtttagtaga acctcgtgaa acttacattt acatatatat aaacttgcat
       61 aaattggtca atgcaagaaa tacatatttg gtcttttcta attcgtagtt tttcaagttc
      121 ttagatgctt tctttttctc ttttttacag atcatcaagg aagtaattat ctactttttc
      181 tctagaagat ctaaacatgt ctcgagctca agcttcgaat tctgcagtcg acggtaccgc
      241 gggcccggga tccccactag tcgccaccat ggcctcctcc gaggacgtca tcaaggagtt
      301 catgcgcttc aaggtgcgca tggagggctc cgtgaacggc cacgagttcg agatcgaggg
      361 cgagggcgag ggccgcccct acgagggcac ccagaccgcc aagctgaagg tgaccaaggg
      421 cggccccctg cccttcgcct gggacatcct gtccccccag ttccagtacg gctccaaggt
      481 gtacgtgaag caccccgccg acatccccga ctacaagaag ctgtccttcc ccgagggctt
      541 caagtgggag cgcgtgatga acttcgagga cggcggcgtg gtgaccgtga cccaggactc
      601 ctccctgcag gacggccgct tcatctacaa ggtgaagttc atcggcgtga acttcccctc
      661 cgacggcccc gtaatgcaga agaagactat gggctgggag ccctccaccg agcgcctgta
      721 cccccgcgac ggcgtgctga agggcgagat ccacaaggcc ctgaagctga aggacggcgg
      781 ccactacctg gtggagttca agtccatcta catggccaag aagcccgtgc agctgcccgg
      841 ctactactac gtggactcca agctggacat cacctcccac aacgaggact acaccatcgt
      901 ggagcagtac gagcgcaccg agggccgcca ccacctgttc ctgtctgatc cctggtcttc
      961 ttcgggagat ctaaa
//
