LOCUS       sequence-130417-        1095 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a biosensor comprised of a fast-maturing pH-stable red fluorescent 
            protein fused to a pH-sensitive green fluorescent protein variant. 
            Cytosolic localization..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1095)
  AUTHORS   Rosado CJ, Mijaljica D, Hatzinisiriou I, Prescott M, Devenish RJ
  TITLE     Rosella: a fluorescent pH-biosensor for reporting vacuolar turnover 
            of cytosol and organelles in yeast.
  JOURNAL   Autophagy. 2008 Feb;4(2):205-13. Epub 2007 Nov 21.
  PUBMED    18094608
REFERENCE   2  (bases 1 to 1095)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Autophagy."; date: "2008-02"; volume: "4(2)"; pages: "205-13. Epub 
            2007 Nov 21"
COMMENT     Sequence Label: pAS1NB c Rosella I
FEATURES             Location/Qualifiers
     source          1..1095
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(142..849)
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKANF
                     KIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLEFV
                     TAAGITQGMDELYK"
     primer_bind     complement(182..202)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     797..825
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     complement(939..962)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 atctatcgat ttcaattcaa ttcaatttat ttcttttcgg ataagaaagc aacacctggc
       61 aattccttac cttccaataa ttccaaagaa gcaccaccac cagtagagac atgggagatc
      121 cgcggccgct cagtgatcag atttgtatag ttcatccatg ccttgtgtaa tcccagcagc
      181 tgttacaaac tcaagaagga ccatgtggtc tctcttttcg ttgggatctt tcgaaagagt
      241 agaagttgta aacaggtaat ggttgtctgg taaaaggacg ggcccatcgc caattggagt
      301 attttgttga taatggtctg ctagttgaac gcctccatct tcaatgttgt gtctaatttt
      361 gaagttagct ttgattccat tcttttgttt gtctgccatg atgtacacct ggtgatcgtt
      421 atagttgtat tccaatttgt gtccaagaat gtttccatct tctttaaaat caataccttt
      481 taactcgatt ctattaacaa gggtatcacc ttcaaacttg acttcagcac gtgtcttgta
      541 gttcccgtca tctttgaaaa atatagttct ttcctgtaca taaccttcgg gcatggcact
      601 cttgaaaaag tcatgccgtt tcatatgatc tgggtatctt gaaaagcatt gaacaccata
      661 agttaaagta gtgacaagtg ttggccaagg aacaggtagt tttccagtag tgcaaataaa
      721 tttaagggta agttttccgt atgttgcatc accttcaccc tctccactga cagaaaattt
      781 gtgcccatta acatcaccat ctaattcaac aagaattggg acaactccag tgaaaagttc
      841 ttctcctttt agatctcccg aagaagacca gggatcagac aggaacaggt ggtggcggcc
      901 ctcggtgcgc tcgtactgct ccacgatggt gtagtcctcg ttgtgggagg tgatgtccag
      961 cttggagtcc acgtagtagt agccgggcag ctgcacgggc ttcttggcca tgtagatgga
     1021 cttgaactcc accaggtagt ggccgccgtc cttcagcttc agggccttgt ggatctcgcc
     1081 cttcagcacg ccgtc
//
