LOCUS       sequence-130425-        1096 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a biosensor comprised of a fast-maturing pH-stable red fluorescent 
            protein fused to a pH-sensitive green fluorescent protein variant. 
            Mitochodrial localization..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1096)
  AUTHORS   Rosado CJ, Mijaljica D, Hatzinisiriou I, Prescott M, Devenish RJ
  TITLE     Rosella: a fluorescent pH-biosensor for reporting vacuolar turnover 
            of cytosol and organelles in yeast.
  JOURNAL   Autophagy. 2008 Feb;4(2):205-13. Epub 2007 Nov 21.
  PUBMED    18094608
REFERENCE   2  (bases 1 to 1096)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Autophagy."; date: "2008-02"; volume: "4(2)"; pages: "205-13. Epub 
            2007 Nov 21"
COMMENT     Sequence Label: pAS1NB m Rosella I
FEATURES             Location/Qualifiers
     source          1..1096
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(143..850)
                     /codon_start=1
                     /product="pH-sensitive mutant of green fluorescent protein 
                     (Ng et al., 2002)"
                     /label=superecliptic pHluorin
                     /note="excited strongly by blue light at neutral pH but 
                     minimally fluorescent at acidic pH"
                     /translation="KGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLKFI
                     CTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTIFFKDDGNY
                     KTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNDHQVYIMADKQKNGIKANF
                     KIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLFTTSTLSKDPNEKRDHMVLLEFV
                     TAAGITQGMDELYK"
     primer_bind     complement(183..203)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     798..826
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     complement(940..963)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gatctatcga tttcaattca attcaattta tttcttttcg gataagaaag caacacctgg
       61 caattcctta ccttccaata attccaaaga agcaccacca ccagtagaga catgggagat
      121 ccgcggccgc tcagtgatca gatttgtata gttcatccat gccttgtgta atcccagcag
      181 ctgttacaaa ctcaagaagg accatgtggt ctctcttttc gttgggatct ttcgaaagag
      241 tagaagttgt aaacaggtaa tggttgtctg gtaaaaggac gggcccatcg ccaattggag
      301 tattttgttg ataatggtct gctagttgaa cgcctccatc ttcaatgttg tgtctaattt
      361 tgaagttagc tttgattcca ttcttttgtt tgtctgccat gatgtacacc tggtgatcgt
      421 tatagttgta ttccaatttg tgtccaagaa tgtttccatc ttctttaaaa tcaatacctt
      481 ttaactcgat tctattaaca agggtatcac cttcaaactt gacttcagca cgtgtcttgt
      541 agttcccgtc atctttgaaa aatatagttc tttcctgtac ataaccttcg ggcatggcac
      601 tcttgaaaaa gtcatgccgt ttcatatgat ctgggtatct tgaaaagcat tgaacaccat
      661 aagttaaagt agtgacaagt gttggccaag gaacaggtag ttttccagta gtgcaaataa
      721 atttaagggt aagttttccg tatgttgcat caccttcacc ctctccactg acagaaaatt
      781 tgtgcccatt aacatcacca tctaattcaa caagaattgg gacaactcca gtgaaaagtt
      841 cttctccttt tagatctccc gaagaagacc agggatcaga caggaacagg tggtggcggc
      901 cctcggtgcg ctcgtactgc tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca
      961 gcttggagtc cacgtagtag tagccgggca gctgcacggg cttcttggcc atgtagatgg
     1021 acttgaactc caccaggtag tggccgccgt ccttcagctt cagggccttg tggatctcgc
     1081 ccttcagcac gccgtc
//
