LOCUS       sequence-13043-e        3348 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3348)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3348)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1149-HQ858693
FEATURES             Location/Qualifiers
     source          1..3348
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(905..1004)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1021..1040)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1022..1040)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1045..1061)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1045..1061)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1163..1182)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1174..1983
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1241..1260)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2130..2718
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2619..2638
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2872..2889
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3048..3075)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3167..3253)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3302..3324
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3316..3333
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3317..3333
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atgggccgct ctccgtgctg cgagaagatg gggctgaaga ggggtccgtg gacggccgag
      181 gaggacagga ttctggtggt gcacgtcgag cgccacggcc acagcaactg gcgcgcgctg
      241 cccaagcagg cgggcctgct ccgctgcggc aagagctgcc gcctccgctg gatcaactac
      301 ctgcgccccg acatcaagcg cggcaacttc agccgggagg aggaggacgc catcatacag
      361 ctccaccaaa tgctcggcaa cagatggtcg acaatcgctg ccaggctgcc ggggaggacg
      421 gacaacgaga tcaagaacgt ctggcacacc cacctcaaga agcgcctgga ggcacagcca
      481 gccagccagc aggcgcccaa gcgcaagccc acgaagaagc agcagcccga gcccgtaacg
      541 acgctcgagg ggccggccgg ggccgtgccg ccggtgtcgc cggagcggtc actctccacg
      601 acgacgtcaa ccaccaccag caccgcggac tactcggccg cctcgtcgct ggagaacgcg
      661 gacgacagct tcacatcgga ggaggactac taccagatcg acgacagctt ctggtccgag
      721 acgctggcga tgacgacgac ggtggacagc ttcgagtccg gggtgcagca ggcggagggc
      781 agcttcggcg cgtcggcggc ggcgccgtcg tcgaccaacg acgacatgga cttctggctc
      841 aagctgttca tgcaggccag cgacatgcag aatttgcccc agattattaa gggtgggcgc
      901 gccgacccag ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt
      961 tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc
     1021 ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct
     1081 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg
     1141 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg
     1201 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat
     1261 aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag
     1321 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga
     1381 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct
     1441 gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa
     1501 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg
     1561 cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag
     1621 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat
     1681 ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat
     1741 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta
     1801 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc
     1861 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat
     1921 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc
     1981 taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac
     2041 ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca
     2101 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     2161 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     2221 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     2281 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     2341 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     2401 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     2461 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     2521 cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     2581 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     2641 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     2701 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     2761 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     2821 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     2881 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     2941 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     3001 gcaattaata cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg
     3061 tcaggatggc cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc
     3121 accctccggg ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca
     3181 ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct
     3241 ttcgttttat ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt
     3301 tcccagtcac gacgttgtaa aacgacggcc agtcttaagc tcgggccc
//
