LOCUS       sequence-130432-         848 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector driving APEX2-OSBP (Human OSBP domain, 
            [marks PI4P] fused to EM peroxidase marker). Also contains 
            bicistronially expressed cytoplasmic red reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 848)
  AUTHORS   Ariotti N, Hall TE, Rae J, Ferguson C, McMahon KA, Martel N, Webb 
            RE, Webb RI, Teasdale RD, Parton RG
  TITLE     Modular Detection of GFP-Labeled Proteins for Rapid Screening by 
            Electron Microscopy in Cells and Organisms.
  JOURNAL   Dev Cell. 2015 Nov 23;35(4):513-25. doi: 
            10.1016/j.devcel.2015.10.016. Epub 2015 Nov 12.
  PUBMED    26585296
REFERENCE   2  (bases 1 to 848)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2015.10.016"; journalName: "Dev Cell"; date: 
            "2015-11-23- 23"; volume: "35"; issue: "4"; pages: "513-25"
COMMENT     Sequence Label: mKate2-P2A-APEX2-OSBP
FEATURES             Location/Qualifiers
     source          1..848
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    34..54
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    356..380
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atacgactca ctatagttct agaggctcga gtgcaactat gtataataaa gttgtcactt
       61 cacagctttg gccttggcca gttccagggc cgtcacccag cgctgccgct caacttctga
      121 actagctttc agatggtagg tctgagcacc cccattggaa atgatgaagt tgcaggagtc
      181 ctccacggtg atgttggctg tggcgaggtt gatggtacca cggcaggtat gtctcatctc
      241 tgcctttgat ctgtagtagc tcaggagccc gttgctcagc acgaaccatc gccgctggta
      301 gcctttgata taattggtcc atttgaagag ccagccctct cgagccgagc ccgaagccac
      361 tttgtacaag aaagctgggt cgacggcatc agcaaaccca agctcggaaa gcttttggtg
      421 agcctcagcg taatcagcaa agaaggcatc ttcgtccgct gcatatttgt caacgagagg
      481 gcggaataca gggtcagaca aaagagcctt gtcagaaggt agctgaagga gaccttcctt
      541 ctcaccactc aacaactccg tgaagtatga gttgtcgaaa ataagaggat tagaggtcca
      601 gggaccctca aatccagaac gctccttgtg tgcagctcca atagtgtgac ccccagatag
      661 agcaacgata tcttggtcag taagccccat agctttgcca aacacatctc tcaaatggtc
      721 agaaccctta gtgggatcgg gcaagcgacc ctctggtggt ggctcaggct tgtcctctct
      781 tccagggtgg aatggaacct taggtccacc cgtgacctca acggcaacaa cgccagccaa
      841 ctggtaga
//
