LOCUS       sequence-130433-         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression vector driving APEX2-ML1N (Mouse 2xML1N domain,
            [marks PI(3,5)P2] fused to EM peroxidase marker). Also contains 
            bicistronially expressed cytoplasmic red reporter..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Ariotti N, Hall TE, Rae J, Ferguson C, McMahon KA, Martel N, Webb 
            RE, Webb RI, Teasdale RD, Parton RG
  TITLE     Modular Detection of GFP-Labeled Proteins for Rapid Screening by 
            Electron Microscopy in Cells and Organisms.
  JOURNAL   Dev Cell. 2015 Nov 23;35(4):513-25. doi: 
            10.1016/j.devcel.2015.10.016. Epub 2015 Nov 12.
  PUBMED    26585296
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2015.10.016"; journalName: "Dev Cell"; date: 
            "2015-11-23- 23"; volume: "35"; issue: "4"; pages: "513-25"
COMMENT     Sequence Label: mKate2-P2A-APEX2-ML1N*2
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    34..54
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     protein_bind    524..548
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atacgactca ctatagttct agaggctcga gtgcaactat gtataataaa gttgttcgga
       61 tccttaatta tccaggtcag gggggtccag catcagttta catggcttgc gccctttggc
      121 ccgaaattta tcgcaagggc tcataaagaa atacttcagc cgccttctca ggtcttcctc
      181 ttcggtaggg gtagtaggtg ctggggatgt tccgacctgg gttccgtagc ctgggttagg
      241 ggtcagcagt ctttcagtct cgcttgctcg tcgtccagct ggagttgcca ttgtcaggat
      301 tctcagcatc agcttacagg gttttcgccc ctttgcgcgg aatttatcgc atggactcat
      361 aaagaagtac ttcagccgtc tcctcaggtc ctcttcctca gtgggtgtgg tgggagctgg
      421 agatgtgccc acttgagtgc cgtatccggg gttgggtgtc agcagccgtt cggtctcgct
      481 tgccctccgt ccagcaggtg ttgccatagt caggtcagag tacaccactt tgtacaagaa
      541 agctgggtcg acggcatcag caaacccaag ctcggaaagc ttttggtgag cctcagcgta
      601 atcagcaaag aaggcatctt cgtccgctgc atatttgtca acgagagggc ggaatacagg
      661 gtcagacaaa agagccttgt cagaaggtag ctgaaggaga ccttccttct caccactcaa
      721 caactccgtg aagtatgagt tgtcgaaaat aagaggatta gaggtccagg gaccctcaaa
      781 tccagaacgc tccttgtgtg cagctccaat agtgtgacc
//
