LOCUS       sequence-130440-         593 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Zebrafish expression vector for ubiquitous expression of APEX2-GBP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 593)
  AUTHORS   Ariotti N, Hall TE, Rae J, Ferguson C, McMahon KA, Martel N, Webb 
            RE, Webb RI, Teasdale RD, Parton RG
  TITLE     Modular Detection of GFP-Labeled Proteins for Rapid Screening by 
            Electron Microscopy in Cells and Organisms.
  JOURNAL   Dev Cell. 2015 Nov 23;35(4):513-25. doi: 
            10.1016/j.devcel.2015.10.016. Epub 2015 Nov 12.
  PUBMED    26585296
REFERENCE   2  (bases 1 to 593)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2015.10.016"; journalName: "Dev Cell"; date: 
            "2015-11-23- 23"; volume: "35"; issue: "4"; pages: "513-25"
COMMENT     Sequence Label: Bact2-APEX2-GBP in pDEST-Tol2-pA2-acrys-cherry
FEATURES             Location/Qualifiers
     source          1..593
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..18
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2..18
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     misc_feature    join(52..63,64..251)
                     /label=Tol2 left end
                     /note="left end of the Tol2 transposon from the medaka fish
                     Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 ctctagaact agtggatctg ctgggcttgc tgaaggtagg gggtcaagaa ccagaggtgt
       61 aaagtacttg agtaatttta cttgattact gtacttaagt attatttttg gggattttta
      121 ctttacttga gtacaattaa aaatcaatac ttttactttt acttaattac atttttttag
      181 aaaaaaaagt actttttact ccttacaatt ttatttacag tcaaaaagta cttatttttt
      241 ggagatcact tcattctatt ttcccttgct attaccaaac caattgaatt gcgctgatgc
      301 ccagtttaat ttaaatgtta tttattctgc ctatgaaaat cgttttcaca ttatatgaaa
      361 ttggtcagac atgttcattg gtcctttgga agtgacgtca tgtcacatct attaccacaa
      421 tgcacagcac cttgacctgg aaattaggga aattataaca gtcaatcagt ggaagaaaat
      481 ggaggaagta tgtgattcat cagcagctgc gagcagcaca gtccaaaatc agccacagga
      541 tcaagagcac ccgtggccgt atcttcgcag atctgatcta gaggatcata atc
//
