LOCUS       sequence-130444-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone containing mKate2-P2A-APEX2 with no stop codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Ariotti N, Hall TE, Rae J, Ferguson C, McMahon KA, Martel N, Webb 
            RE, Webb RI, Teasdale RD, Parton RG
  TITLE     Modular Detection of GFP-Labeled Proteins for Rapid Screening by 
            Electron Microscopy in Cells and Organisms.
  JOURNAL   Dev Cell. 2015 Nov 23;35(4):513-25. doi: 
            10.1016/j.devcel.2015.10.016. Epub 2015 Nov 12.
  PUBMED    26585296
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2015.10.016"; journalName: "Dev Cell"; date: 
            "2015-11-23- 23"; volume: "35"; issue: "4"; pages: "513-25"
COMMENT     Sequence Label: pME-mKate-P2A-APEX2-NS
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    74..98
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aataatgatt ttattttgac tgatagtgac ctgttcgttg caacaaattg ataagcaatg
       61 ctttcttata atgccaactt tgtacaagaa agctgggtcg acggcatcag caaacccaag
      121 ctcggaaagc ttttggtgag cctcagcgta atcagcaaag aaggcatctt cgtccgctgc
      181 atatttgtca acgagagggc ggaatacagg gtcagacaaa agagccttgt cagaaggtag
      241 ctgaaggaga ccttccttct caccactcaa caactccgtg aagtatgagt tgtcgaaaat
      301 aagaggatta gaggtccagg gaccctcaaa tccagaacgc tccttgtgtg cagctccaat
      361 agtgtgaccc ccagatagag caacgatatc ttggtcagta agccccatag ctttgccaaa
      421 cacatctctc aaatggtcag aacccttagt gggatcgggc aagcgaccct ctggtggtgg
      481 ctcaggcttg tcctctcttc cagggtggaa tggaacctta ggtccacccg tgacctcaac
      541 ggcaacaacg ccagccaact ggtagaaatc ggcgtagctc aaaataggga actccgcctt
      601 gagtggctcc aaaagcctaa cagcgatgtc aagaccgttg ttagcgctgt gagccagttc
      661 ggcagggtgc ttgatggttc cgaagggtcc accggtcttc gtgcccttgt caaaggttcc
      721 agcagagtgg aatgccaaac ggagcattag aggagcgcat ctcttctcag cgatgaagcc
      781 tctgagcttc ttcttcgcct tctcaacggc gtcctggtaa tcagcactca cagttg
//
