LOCUS       sequence-130445-         854 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Multisite gateway entry clone for adding C-terminal fusions of 
            APEX2-P2A-mKate2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 854)
  AUTHORS   Ariotti N, Hall TE, Rae J, Ferguson C, McMahon KA, Martel N, Webb 
            RE, Webb RI, Teasdale RD, Parton RG
  TITLE     Modular Detection of GFP-Labeled Proteins for Rapid Screening by 
            Electron Microscopy in Cells and Organisms.
  JOURNAL   Dev Cell. 2015 Nov 23;35(4):513-25. doi: 
            10.1016/j.devcel.2015.10.016. Epub 2015 Nov 12.
  PUBMED    26585296
REFERENCE   2  (bases 1 to 854)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2015.10.016"; journalName: "Dev Cell"; date: 
            "2015-11-23- 23"; volume: "35"; issue: "4"; pages: "513-25"
COMMENT     Sequence Label: p3E-APEX2-P2A-mKate2
FEATURES             Location/Qualifiers
     source          1..854
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    32..127
                     /gene="mutant version of attL"
                     /label=attL3
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(133..828)
                     /codon_start=1
                     /product="monomeric far-red fluorescent protein (Shcherbo 
                     et al., 2009)"
                     /label=mKate2
                     /note="mammalian codon-optimized"
                     /translation="VSELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIKA
                     VEGGPLPFAFDILATSFMYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
                     TQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEASTETLYPADGGLEGRADMALK
                     LVGGGHLICNLKTTYRSKKPAKNLKMPGVYYVDRRLERIKEADKETYVEQHEVAVARYC
                     DLPSKLGHR"
ORIGIN
        1 cgcggccgcg ttaacgctac catggagctc caaataatga ttttattttg actgatagtg
       61 acctgttcgt tgcaacaaat tgataagcaa tgctttttta taatgccaac tttgtataat
      121 aaagttgact agtcatctgt gccccagttt gctagggagg tcgcagtatc tggccacagc
      181 cacctcgtgc tgctcgacgt aggtctcttt gtcggcctcc ttgattcttt ccagtcttct
      241 gtccacatag tagacgccgg gcatcttgag gttcttagcg ggtttcttgg atctgtatgt
      301 ggtcttcaag ttgcagatca ggtggccccc gcccacgagc ttcagggcca tgtcggctct
      361 gccttccagg ccgccgtcag cggggtacag ggtctcggtg gaggcctccc agccgagtgt
      421 tttcttctgc atcacagggc cgttggatgg gaagttcacc cctctgatct tgacgttgta
      481 gatgaggcag ccgtcctgga ggctggtgtc ctgggtagcg gtcagcacgc ccccgtcttc
      541 gtatgtggtg actctctccc atgtgaagcc ctcggggaag gactgcttaa agaagtcggg
      601 gatgccctgg gtgtggttga tgaaggtttt gctgccgtac atgaagctgg tagccaggat
      661 gtcgaaggcg aaggggagag ggccgccctc gaccgccttg attctcatgg tctgggtgcc
      721 ctcgtagggc ttgccttcgc cctcggatgt gcacttgaag tggtggttgt tcacggtgcc
      781 ctccatgtac agcttcatgt gcatgttctc cttaatcagc tcgctcacgg taccgcttcc
      841 aggagcagga ccgg
//
