LOCUS       sequence-130447-         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Sleeping Beauty (SB) transposon with G418 resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Chicaybam L, Barcelos C, Peixoto B, Carneiro M, Limia CG, Redondo P,
            Lira C, Paraguassu-Braga F, Vasconcelos ZF, Barros L, Bonamino MH
  TITLE     An Efficient Electroporation Protocol for the Genetic Modification 
            of Mammalian Cells.
  JOURNAL   Front Bioeng Biotechnol. 2017 Jan 23;4:99. doi: 
            10.3389/fbioe.2016.00099. eCollection 2016.
  PUBMED    28168187
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Front 
            Bioeng Biotechnol."; date: "2017-01-23"; volume: "4:99. doi"; pages:
            " 10.3389/fbioe.2016.00099. eCollection 2016"
COMMENT     Sequence Label: pT3-Neo
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(353..373)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
ORIGIN
        1 gtcggaagtt tacatacact taagttggag tcattaaaac tcgtttttca actactccac
       61 aaatttcttg ttaacaaaca atagttttgg caagtcagtt aggacatcta ctttgtgcat
      121 gacacaagtc atttttccaa caattgttta cagacagatt atttcactta taattcactg
      181 tatcacaatt ccagtgggtc agaagtttac atacactaag ttgactgtgc ctttaaacag
      241 cttggaaaat tccagaaaat gatgtcatgg ctttagaagc ttctgataga ctaattgaca
      301 tcatttgagt caattggagg tgtacctgtg gatgtatttc aaggaattct gtggaatgtg
      361 tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
      421 catatcgata ctagtttaat taagatctcg agggaatgaa agaccccacc tgtaggtttg
      481 gcaagctagc ttaagtaacg ccattttgca aggcatggaa aatacataac tgagaataga
      541 gaagttcaga tcaaggttag gaacagagag acagcagaat atgggccaaa caggatatct
      601 gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc
      661 cgccctcagc agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa
      721 tgaccctgtg ccttatttga actaaccaat cagttcgctt
//
