LOCUS       sequence-130448-         657 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway entry clone containing BTK-PH with no stop codon.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 657)
  AUTHORS   Ariotti N, Hall TE, Rae J, Ferguson C, McMahon KA, Martel N, Webb 
            RE, Webb RI, Teasdale RD, Parton RG
  TITLE     Modular Detection of GFP-Labeled Proteins for Rapid Screening by 
            Electron Microscopy in Cells and Organisms.
  JOURNAL   Dev Cell. 2015 Nov 23;35(4):513-25. doi: 
            10.1016/j.devcel.2015.10.016. Epub 2015 Nov 12.
  PUBMED    26585296
REFERENCE   2  (bases 1 to 657)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2015.10.016"; journalName: "Dev Cell"; date: 
            "2015-11-23- 23"; volume: "35"; issue: "4"; pages: "513-25"
COMMENT     Sequence Label: pME-BTK-PH
FEATURES             Location/Qualifiers
     source          1..657
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    10..109
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     regulatory      638..647
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 gctggatggc aaataatgat tttattttga ctgatagtga cctgttcgtt gcaacaaatt
       61 gataagcaat gctttcttat aatgccaact ttgtacaaga aagctgggtc cggttttaag
      121 cttccattcc tgttctccaa aatttggcag cccatagcat ttttggctgt ctgagagcag
      181 cagagatact gcccatcgat ccagaagcaa gggtgatatt tctgaaccag atcactgttg
      241 taccggatta cgtttttgag ctggtgaatc caccgcttcc ttagttcttc agttggggag
      301 aagacgtaga gaggcccttc atcatataca acctggaagg gataagggaa cctttcaatg
      361 attgaaattt gctccatttc actggactct tcacctcttc tcggaatctg tctttctgga
      421 ggaggatttt tttcaggaac cactgtttca acacaagtga tcttctcaac atctattgaa
      481 cccttcttac tgcctcttct cccacgttca aagtcatact catagtagga gagtttgtgc
      541 acggtcaaga gaaacaggcg cttcttgaag tttagaggtg atgttttctt tttctgttgg
      601 gatcgcttca gaaagatgct ctccagaatc actgcggcca tggtggcagc ctgcttt
//
