LOCUS       sequence-130450-         754 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial inducible gRNA expression plasmid.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 754)
  TITLE     Bacterial constitutive gRNA expression plasmid
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pFG018
FEATURES             Location/Qualifiers
     source          1..754
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_RNA        40..115
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
ORIGIN
        1 ctctctactg tttctccatt cgcaagatgt ggcgtgttag ttttagagct agaaatagca
       61 agttaaaata aggctagtcc gttatcaact tgaaaaagtg gcaccgagtc ggtgctttta
      121 aaaaaaaaac cccctgtcag gggcggggtt tttttttaca agtctcagtg tgctgaagtt
      181 ttgggaccat tctccatggg tatggacagt tttccctttg atatgtaacg gtgaacagtt
      241 gttctacttt tgtttgttag tcttgatgct tcactgatag atacaagagc cataagaacc
      301 tcagatcctt ccgtatttag ccagtatgtt ctctagtgtg gttcgttgtt tttgcgtgag
      361 ccatgagaac gaaccattga gatcatactt actttgcatg tcactcaaaa attttgcctc
      421 aaaactggtg agctgaattt ttgcagttaa agcatcgtgt agtgtttttc ttagtccgtt
      481 atgtaggtag gaatctgatg taatggttgt tggtattttg tcaccattca tttttatctg
      541 gttgttctca agttcggtta cgagatccat ttgtctatct agttcaactt ggaaaatcaa
      601 cgtatcagtc gggcggcctc gcttatcaac caccaatttc atattgctgt aagtgtttaa
      661 atctttactt attggtttca aaacccattg gttaagcctt ttaaactcat ggtagttatt
      721 ttcaagcatt aacatgaact taaattcatc aagg
//
