LOCUS       sequence-130464-         735 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Egg cell-specific promoter-controlled expression of 
            3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target
            sequence (U6-26 promoter), Hyg resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 735)
  AUTHORS   Wang ZP, Xing HL, Dong L, Zhang HY, Han CY, Wang XC, Chen QJ
  TITLE     Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently 
            generates homozygous mutants for multiple target genes in 
            Arabidopsis in a single generation.
  JOURNAL   Genome Biol. 2015 Jul 21;16:144. doi: 10.1186/s13059-015-0715-0.
  PUBMED    26193878
REFERENCE   2  (bases 1 to 735)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-07-21"; volume: "16:144. doi"; pages: " 
            10.1186/s13059-015-0715-0"
COMMENT     Sequence Label: pHEE401E
FEATURES             Location/Qualifiers
     source          1..735
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(305..325)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(332..397)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 cgtcaacctt cgccatctcg ttggagaaaa tctcctgcag gtagcagatg cgattcttcc
       61 tgcgcgtgta ccgcctgcgg gcggtgcgct tgagccgcgt agcctcagcc gtctcgccgc
      121 tgtcgaacag gagagcgcca atgagattct tcttgatgga atgccgatcg gtgttgccca
      181 ggaccttgaa cttctttgag ggcaccttgt actcgtcggt gatcacggcc cagccaacag
      241 agttagtccc aatatcgagg ccgatcgagt acttcttgtc agcagctggc accccgtgga
      301 tgccaacctt cctcttcttc ttcggagcca tcttgtcatc atcatccttg taatcaatgt
      361 cgtggtcctt gtaatccccg tcgtggtcct tgtaatccat ctagatttct caacagattg
      421 ataaggtcga aagaagaaag agtagaagtt gctttggttg atttgtttgt gatgagaatt
      481 gtgtgttaac tttagagagt ttatatagag acagtagaga cttgactgtt acaaataaat
      541 ggtagagtga aagcccaagt aattacgagg gaagctcatt agattgtgag ataattagtg
      601 cttagtggtt tagtgggtaa actaggtgta attactctat taaaagctgc gtaattagtg
      661 ggtctgttta ggggctaatg ggccggatta gaatcactca gtctgaaact cgtttgattt
      721 agttcaatag aacca
//
