LOCUS       sequence-130469-         897 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses NLS-dCas9-GFP11x7-NLS in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 897)
  AUTHORS   Kamiyama D, Sekine S, Barsi-Rhyne B, Hu J, Chen B, Gilbert LA, 
            Ishikawa H, Leonetti MD, Marshall WF, Weissman JS, Huang B
  TITLE     Versatile protein tagging in cells with split fluorescent protein.
  JOURNAL   Nat Commun. 2016 Mar 18;7:11046. doi: 10.1038/ncomms11046.
  PUBMED    26988139
REFERENCE   2  (bases 1 to 897)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-03-18"; volume: "7:11046. doi"; pages: " 
            10.1038/ncomms11046"
COMMENT     Sequence Label: pHRm-NLS-dCas9-GFP11x7-NLS
FEATURES             Location/Qualifiers
     source          1..897
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ttcgctcagg ccgcctctct cggccttggt cagattgtcg aacttcctct gggtaatcag
       61 cttggcattc agcagctggc gccagtagtt cttcatcttc ttcacgacct cttcggaggg
      121 cacgttgtcg ctcttgcccc ggttcttgtc gctccgagtc agcactttgt tatcgatgga
      181 gtcgtccttc agaaagctct gaggcacgat agcgtccaca tcgtagtcgg acagccggtt
      241 gatgtccagt tcctggtcca cgtacatatc ccgcccattc tgcaggtagt acaggtacag
      301 cttctcgttc tgcagctggg tgttttccac ggggtgttct ttcaggatct ggctgcccag
      361 ctctttgatg ccctcttcga tccgcttcat tctctcgcgg ctgttcttct gtcccttctg
      421 ggtggtctgg ttctctctgg ccatttcgat cacgatgttc tcgggcttgt gccggcccat
      481 cactttcacg agctcgtcca ccaccttcac tgtctgcagg atgcccttct taatggcggg
      541 gctgccggcc agattggcaa tgtgctcgtg caggctatcg ccctggccgg acacctgggc
      601 tttctggatg tcctctttaa aggtcaggct gtcgtcgtgg atcagctgca tgaagtttct
      661 gttggcgaag ccgtcggact tcaggaaatc caggattgtc ttgccggact gcttgtcccg
      721 gatgccgttg atcagcttcc ggctcagcct gccccagccg gtgtatctcc gccgcttcag
      781 ctgcttcatc actttgtcgt cgaacaggtg ggcataggtt ttcagccgtt cctcgatcat
      841 ctctctgtcc tcaaacagtg tcagggtcag cacgatatct tccagaatgt cctcgtt
//
