LOCUS       sequence-130475-         870 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  MESA target chain with dTomato ectodomain, no linker domain, M 
            cleavage sequence, and tTA-BFP fusion .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 870)
  AUTHORS   Daringer NM, Dudek RM, Schwarz KA, Leonard JN
  TITLE     Modular Extracellular Sensor Architecture for Engineering Mammalian 
            Cell-based Devices.
  JOURNAL   ACS Synth Biol. 2014 Mar 11.
  PUBMED    24611683
REFERENCE   2  (bases 1 to 870)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2014 Mar 11."
COMMENT     Sequence Label: dTomato_LD 0_CS M_tTA-BFP
FEATURES             Location/Qualifiers
     source          1..870
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             77..775
                     /codon_start=1
                     /product="dimeric variant of DsRed fluorescent protein 
                     (Shaner et al., 2004)"
                     /label=dTomato
                     /note="mammalian codon-optimized"
                     /translation="VSKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGL
                     VTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQ
                     ALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHH
                     LFLYGMDELYK"
     primer_bind     complement(207..225)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     475..494
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     669..692
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
ORIGIN
        1 gtgccgagcc atctctctta ggcgcttgct gctgctgctg ctgcagctgt cacaactcct
       61 agctgtcact caaggggtga gcaagggcga ggaggtcatc aaagagttca tgcgcttcaa
      121 ggtgcgcatg gagggctcca tgaacggcca cgagttcgag atcgagggcg agggcgaggg
      181 ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggcg gccccctgcc
      241 cttcgcctgg gacatcctgt ccccccagtt catgtacggc tccaaggcgt acgtgaagca
      301 ccccgccgac atccccgatt acaagaagct gtccttcccc gagggcttca agtgggagcg
      361 cgtgatgaac ttcgaggacg gcggtctggt gaccgtgacc caggactcct ccctgcagga
      421 cggcacgctg atctacaagg tgaagatgcg cggcaccaac ttcccccccg acggccccgt
      481 aatgcagaag aagaccatgg gctgggaggc ctccaccgag cgcctgtacc cccgcgacgg
      541 cgtgctgaag ggcgagatcc accaggccct gaagctgaag gacggcggcc actacctggt
      601 ggagttcaag accatctaca tggccaagaa gcccgtgcaa ctgcccggct actactacgt
      661 ggacaccaag ctggacatca cctcccacaa cgaggactac accatcgtgg aacagtacga
      721 gcgctccgag ggccgccacc acctgttcct gtacggcatg gacgagctgt acaagaagct
      781 tttttgggca ctggtcgtgg ttgctggagt cctgttttgt tatggcttgc tagtgacagt
      841 ggctctttgt gttgaaaacc tgtattttca
//
