LOCUS       sequence-130479-         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway ORF clone of human RAC1 [NM_006908.4] with stop codon (for 
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E207 Hs.RAC1
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    86..185
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(184..762)
                     /codon_start=1
                     /product="human Rac family small GTPase 1"
                     /label=Rac1
                     /translation="MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVM
                     VDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHH
                     CPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQR
                     GLKTVFDEAIRAVLCPPPVKKRKRKCLLL"
     protein_bind    complement(765..864)
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
ORIGIN
        1 cggtgtgcgt cacccggcaa ccttgggtag cagcgaagtc gaggcatttc tgtcctggct
       61 ggtctagagg atctaagctt gcatgcaaat aatgatttta ttttgactga tagtgacctg
      121 ttcgttgcaa caaattgata agcaatgctt tcctataatg ccaactttgt acaagaaagt
      181 tgattacaac agcaggcatt ttctcttcct cttcttcacg ggaggcgggc agaggactgc
      241 tcggatcgct tcgtcaaaca ctgtcttgag gcctcgctgt gtgagcgccg agcactccag
      301 gtattttaca gcaccaatct ccttagccat ggctagaccc tgcggatagg tgatgggagt
      361 cagcttcttc tccttcagtt tctcgatcgt gtctttatca tccctaagat caagtttagt
      421 tcccactagg atgatgggag tgttgggaca gtggtgccgc acctcaggat accactttgc
      481 acggacattt tcaaatgatg caggactcac aagggaaaag caaattaaga acacatctgt
      541 ttgcggatag gatagggggc gtaatctgtc ataatcttct tgtccagctg tatcccataa
      601 gcccagattc accggttttc catctaccat aacattggca gaataattgt caaagacagt
      661 agggatatat tctccaggaa atgcattggt tgtgtaactg atcagtaggc aagttttacc
      721 tacagctccg tctcccacca ccacacactt gatggcctgc atggtgccaa cttttttgta
      781 caaagttggc attataaaaa agcattgctt atcaatttgt tgcaacgaac aggtcactat
      841 cagtcaaaat aaaatcatta tttc
//
