LOCUS       sequence-130480-         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway ORF clone of human RAC1 [NM_006908.4] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E208 Hs.RAC1-nostop
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    86..185
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(184..759)
                     /codon_start=1
                     /product="human Rac family small GTPase 1"
                     /label=Rac1
                     /translation="MQAIKCVVVGDGAVGKTCLLISYTTNAFPGEYIPTVFDNYSANVM
                     VDGKPVNLGLWDTAGQEDYDRLRPLSYPQTDVFLICFSLVSPASFENVRAKWYPEVRHH
                     CPNTPIILVGTKLDLRDDKDTIEKLKEKKLTPITYPQGLAMAKEIGAVKYLECSALTQR
                     GLKTVFDEAIRAVLCPPPVKKRKRKCLLL"
     protein_bind    complement(762..786)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cggtgtgcgt cacccggcaa ccttgggtag cagcgaagtc gaggcatttc tgtcctggct
       61 ggtctagagg atctaagctt gcatgcaaat aatgatttta ttttgactga tagtgacctg
      121 ttcgttgcaa caaattgata agcaatgctt tcctataatg ccaactttgt acaagaaagt
      181 tgacaacagc aggcattttc tcttcctctt cttcacggga ggcgggcaga ggactgctcg
      241 gatcgcttcg tcaaacactg tcttgaggcc tcgctgtgtg agcgccgagc actccaggta
      301 ttttacagca ccaatctcct tagccatggc tagaccctgc ggataggtga tgggagtcag
      361 cttcttctcc ttcagtttct cgatcgtgtc tttatcatcc ctaagatcaa gtttagttcc
      421 cactaggatg atgggagtgt tgggacagtg gtgccgcacc tcaggatacc actttgcacg
      481 gacattttca aatgatgcag gactcacaag ggaaaagcaa attaagaaca catctgtttg
      541 cggataggat agggggcgta atctgtcata atcttcttgt ccagctgtat cccataagcc
      601 cagattcacc ggttttccat ctaccataac attggcagaa taattgtcaa agacagtagg
      661 gatatattct ccaggaaatg cattggttgt gtaactgatc agtaggcaag ttttacctac
      721 agctccgtct cccaccacca cacacttgat ggcctgcatg gtgccaactt ttttgtacaa
      781 agttggcatt ataaaaaagc attgcttatc aatttgttgc aacgaacagg tcactatcag
      841 tcaaaa
//
