LOCUS       sequence-130481-        1123 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  plasmid expressing fusion construct of Gal4 DBD and VP16 AD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1123)
  AUTHORS   Budzynski MA, Puustinen MC, Joutsen J, Sistonen L
  TITLE     Uncoupling Stress-Inducible Phosphorylation of Heat Shock Factor 1 
            from Its Activation.
  JOURNAL   Mol Cell Biol. 2015 Jul;35(14):2530-40. doi: 10.1128/MCB.00816-14. 
            Epub 2015 May 11.
  PUBMED    25963659
REFERENCE   2  (bases 1 to 1123)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00816-14"; journalName: "Mol Cell Biol"; date: 
            "2015-07"; volume: "35"; issue: "14"; pages: "2530-40"
COMMENT     Sequence Label: Gal4-VP16
FEATURES             Location/Qualifiers
     source          1..1123
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             536..769
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
ORIGIN
        1 agycactmga tgccagwtct cgctcrcwtc gagtgtgaga ggcttttttg gagamcaagc
       61 ttgagcagcc tctgtkgkgg cttctgtctt ctatcgaaca agcatgcgat aaggccgact
      121 taaaaagctc aagtgctcca aagaaaaacc gaagtgcgcc aagtgtctga agaacaactg
      181 ggagtgtcgc tactctccca aaaccaaaag gtctccgctg actagggcac atctgacaga
      241 agtggaatca aggctagaaa gactggaaca gctatttcta ctgatttttc ctcgagaaga
      301 ccttgacatg attttgaaaa tggattcttt acaggatata aaagcattgt taacaggatt
      361 atttgtacaa gataatgtga ataaagatgc cgtcacagat agattggctt cagtggagac
      421 tgatatgcct ctaacattga gacagcatag aataagtgcg acatcatcat cggaagagag
      481 tagtaacaaa ggtcaaagac agttgactgt atcgccggaa ttcccgggga tctgggcccc
      541 cccgaccgat gtcagcctgg gggacgagct ccacttagac ggcgaggacg tggcgatggc
      601 gcatgccgac gcgctagacg atttcgatct ggacatgttg ggggacgggg attccccggg
      661 tccgggattt accccccacg actccgcccc ctacggcgct ctggatatgg ccgacttcga
      721 gtttgagcag atgtttaccg atgcccttgg aattgacgag tacggtgggt agggggcgcg
      781 accggacccg catcccccgt ctgggttttc ccctcccgtc cccggttcgt atccacaata
      841 aacacgagca catacattac aaaaacctgc ggttgtcgtc tgattatttg gtggatccgg
      901 gggagatggg ggaggctaac tgaacacgga agagacatac cggaaggaac ccgcgctatg
      961 acggcaataa aaagacagat aaaacgcacg gtgttgggtc gtttgttcma taaacgcggg
     1021 gttcggtccc agggcttgcm mctctgtcga tacccaccga gacccaattg ggaccatacg
     1081 ccgcrgtttc tcctaccgac cacccctaaa tttcggawtg acg
//
