LOCUS       sequence-13049-e        3894 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1155-HQ858699
FEATURES             Location/Qualifiers
     source          1..3894
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1451..1550)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1567..1586)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1568..1586)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1591..1607)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1591..1607)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1709..1728)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1720..2529
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1787..1806)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2676..3264
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3165..3184
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3418..3435
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3594..3621)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3713..3799)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3848..3870
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3862..3879
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3863..3879
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atgccaccgt caacctattt catgacagct agatcagatc acaatttaaa tttcagtgtg
      181 catgaacagg attccactat gcttccaaac gaacagttga cctatgctgg tgctggccag
      241 attgatctat tacctgctga gactctatct agggataagc ttcctgaaaa tcttctggag
      301 cttcaaggac ttcagaatat cagcaaccta cagagtaatt tagtggcccc aggggtgttt
      361 cagcacaatt taacacctgg ggtctttcat ccacagttga acactcctgg actttcagaa
      421 ctgcctcatg ccttatctag ttcaattgac agtaatggaa gtgaagtttc tgcttttctc
      481 gctgatctaa acgctgtttc ttcagcgcca actttatgtt ccacattcca aaacgcttct
      541 tcgttcatgg aaccagtgaa tctagaagct ttcagtattc aaggcgcaca aagtgattgt
      601 gttttgaaca gaacaagtca tccgaatggg aatatctcag tatgtgacag tgctgccttg
      661 gcatcactac atgatagcaa ggagtttatc agtggtagtc tcccttcttt tgctagtgtc
      721 caggaaacaa acctagctgc tagtggcttc aagactcaga agcaggagca aaatgcagtg
      781 tgcaatatcc ctactttcac tgcacggaat caaatgtcag ttgctgcaat gcaaggagca
      841 cttgtccctc aaaagattcc ttcacggatc aatgataata aaagtgaggg tcctgttagc
      901 catccttctg atgtacaaac tcaaccaaat tcagctgtta atggtgttgg tgtgaaacca
      961 cgagtaaggg ctcgtcgtgg acaagcaact gatcctcata gtattgctga acggcttcgc
     1021 agagagaaaa tttcagatag gatgaaaaat ctgcaggatc ttgtcccaaa ttccaataag
     1081 gcagacaagg catccatgct ggatgaaatt attgattatg tgaagtttct tcagcttcag
     1141 gtcaaggtct tgagcatgag tagggtagga gctccaggag cagttcttcc ccttctcaca
     1201 gaatctaaga ctgagggtta ccacggccaa cctctgccac aaggattact ggacgcacta
     1261 gactcggaag acagcttcgt cttcgaggaa gaggtcgtga agctaatgga gaccagcatc
     1321 accagcgcga tgcagtacct tcagagcaag gggctctgcc tgatgccagt cgcccttgct
     1381 tccgcaatat ccacccagaa aggtgtgtcc gctgctgcag tccctcctga acggaagggt
     1441 gggcgcgccg acccagcttt cttgtacaaa gttggcatta taagaaagca ttgcttatca
     1501 atttgttgca acgaacaggt cactatcagt caaaataaaa tcattatttg ccatccagct
     1561 gatatcccct atagtgagtc gtattacatg gtcatagctg tttcctggca gctctggccc
     1621 gtgtctcaaa atctctgatg ttacattgca caagataaaa atatatcatc atgaacaata
     1681 aaactgtctg cttacataaa cagtaataca aggggtgtta tgagccatat tcaacgggaa
     1741 acgtcgaggc cgcgattaaa ttccaacatg gatgctgatt tatatgggta taaatgggct
     1801 cgcgataatg tcgggcaatc aggtgcgaca atctatcgct tgtatgggaa gcccgatgcg
     1861 ccagagttgt ttctgaaaca tggcaaaggt agcgttgcca atgatgttac agatgagatg
     1921 gtcagactaa actggctgac ggaatttatg cctcttccga ccatcaagca ttttatccgt
     1981 actcctgatg atgcatggtt actcaccact gcgatccccg gaaaaacagc attccaggta
     2041 ttagaagaat atcctgattc aggtgaaaat attgttgatg cgctggcagt gttcctgcgc
     2101 cggttgcatt cgattcctgt ttgtaattgt ccttttaaca gcgatcgcgt atttcgtctc
     2161 gctcaggcgc aatcacgaat gaataacggt ttggttgatg cgagtgattt tgatgacgag
     2221 cgtaatggct ggcctgttga acaagtctgg aaagaaatgc ataaactttt gccattctca
     2281 ccggattcag tcgtcactca tggtgatttc tcacttgata accttatttt tgacgagggg
     2341 aaattaatag gttgtattga tgttggacga gtcggaatcg cagaccgata ccaggatctt
     2401 gccatcctat ggaactgcct cggtgagttt tctccttcat tacagaaacg gctttttcaa
     2461 aaatatggta ttgataatcc tgatatgaat aaattgcagt ttcatttgat gctcgatgag
     2521 tttttctaat cagaattggt taattggttg taacactggc agagcattac gctgacttga
     2581 cgggacggcg caagctcatg accaaaatcc cttaacgtga gttacgcgtc gttccactga
     2641 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     2701 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     2761 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     2821 gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     2881 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     2941 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     3001 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     3061 cgtgagcatt gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     3121 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     3181 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     3241 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     3301 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     3361 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     3421 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     3481 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     3541 cgcaacgcaa ttaatacgcg taccgctagc caggaagagt ttgtagaaac gcaaaaaggc
     3601 catccgtcag gatggccttc tgcttagttt gatgcctggc agtttatggc gggcgtcctg
     3661 cccgccaccc tccgggccgt tgcttcacaa cgttcaaatc cgctcccggc ggatttgtcc
     3721 tactcaggag agcgttcacc gacaaacaac agataaaacg aaaggcccag tcttccgact
     3781 gagcctttcg ttttatttga tgcctggcag ttccctactc tcgcgttaac gctagcatgg
     3841 atgttttccc agtcacgacg ttgtaaaacg acggccagtc ttaagctcgg gccc
//
