LOCUS       sequence-130495-        1168 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  plasmid expressing fusion construct of Gal4 BDB VP16 AD and 
            HSF1-Delta∼PRD RD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1168)
  AUTHORS   Budzynski MA, Puustinen MC, Joutsen J, Sistonen L
  TITLE     Uncoupling Stress-Inducible Phosphorylation of Heat Shock Factor 1 
            from Its Activation.
  JOURNAL   Mol Cell Biol. 2015 Jul;35(14):2530-40. doi: 10.1128/MCB.00816-14. 
            Epub 2015 May 11.
  PUBMED    25963659
REFERENCE   2  (bases 1 to 1168)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00816-14"; journalName: "Mol Cell Biol"; date: 
            "2015-07"; volume: "35"; issue: "14"; pages: "2530-40"
COMMENT     Sequence Label: Gal4-VP16-HSF1-Delta∼PRD
FEATURES             Location/Qualifiers
     source          1..1168
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             110..550
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKXLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
ORIGIN
        1 taaaaaaagg ccctwccata akwgtagwtk cwgwwgyctc tkgstaacwt tcmgartrtg
       61 aagaggcttt tttggaggag atctaagctt gaagcaagcc tcctgaaaga tgaagstact
      121 gtcttctatc gaacaagcat gcgatatttg ccgacttaaa aagctcaagt gctccaaaga
      181 aaaaccgaag tgcgccaagt gtctgaagaa caactgggag tgtcgctact ctcccaaaac
      241 caaaaggtct ccgctgacta gggcacatct gacagaagtg gaatcaaggc tagaaagact
      301 ggaacagcta tttctactga tttttcctcg agaagacctt gacatgattt tgaaaatgga
      361 ttctttacag gatataaaag cattgttaac aggattattt gtacaagata atgtgaataa
      421 agatgccgtc acagatagat tggcttcagt ggagactgat atgcctctaa cattgagaca
      481 gcatagaata agtgcgacat catcatcgga agagagtagt aacaaaggtc aaagacagtt
      541 gactgtatcg ccggaattcc attccatgcc caagtatagc cggcagttcg ccctggagca
      601 cgtccacggc tcgggcccct actcggcccc ctccccagcc tacagcagct ccagcctcta
      661 cgcccctgat gctgtggcca gctctggacc catcatctcc gacatcaccg agctggctcc
      721 tgccagcccc atggcctccc ccggcgggag catagacgag aggcccctat ccagcgcccc
      781 cctggtgcgt gtcaaggagg agccccccgc cccgcctcag gccccccggg tagaggaggc
      841 ggctcccggg cgcccagctg ccgtggacgc cctcttggcc ccgaccgccc tcattgactc
      901 catcctgcgg gaggctgaac ctgcccccgc cgccgtcaca gccctcacgg acgccagggg
      961 ccacacggac accgagggcg gctccgcacc ccgcccgcgc gccctgaaag tgctcagcgt
     1021 agcctgctga tccgggatct ggccccccga ccgatgtcag ctgggggacg agctcactag
     1081 acggsaagac gtgcgatggc gcatggcgaa cgcgctagac gatccgaatc tgamtgtggg
     1141 gacggattcc cggaccggaa taatmccc
//
