LOCUS       sequence-13050-e        3588 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3588)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3588)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1156-HQ858700
FEATURES             Location/Qualifiers
     source          1..3588
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1145..1244)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1261..1280)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1262..1280)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1285..1301)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1285..1301)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1403..1422)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1414..2223
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1481..1500)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2370..2958
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2859..2878
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3112..3129
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3288..3315)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3407..3493)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3542..3564
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3556..3573
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3557..3573
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atggacagca ggagggagtc ctcggagacc ttgaggaaca aatgcgcagc ctgcttcagg
      181 cagtacaaca ggatggagca cctggtggag cacatgaagg tctcgtacca ctcggtgcac
      241 gagcccaggt gcggcgtctg cgggaagcac tgccgctcct tggagtcgct cagggagcat
      301 ctcatcgggc cgttgcccaa gatggagtgc gcgcgggtct tcggcgtccg cggctgcagc
      361 atctgcctca acgttctcga cagcagcgcc gccgtcagat accaccgtgc ggcctgccag
      421 tactctcgtg ctgctccgat gcccaggggc ggtagcatga ctgggcgcgc ggtcgccctg
      481 gcttgcaaga tggtaggggg aggaagcgac ggctccatgg acctttgtgc gagggtgtgc
      541 ctcgttggag aagatgagca cgtcatcttc cagacctatg tcaaacctac actccctgtc
      601 acgaactaca ggtatgaagt gactgggata aggccagagt acctgaggga cgcaatgccg
      661 ctcaaggttg cgcagagaag aatccaggaa atcctgtgca acggggagtc actgtggaag
      721 ttacgcccaa gaagctatgg tagggcaaag gtactcgttg gtcatggcct cgaccatgac
      781 cttgagcgcc tagggttaga gtacccggca ttcatgatca gggatactgc aaaataccca
      841 ccactaatga agactagcaa gctgagtaac tccctcaagt accttacaca agcatacctc
      901 gggtatgaca tccatactgg cattcaggac ccctacgagg actgcgtcgc aacaatgagg
      961 ctgtacatca ggatgagatc ncaggctcnc cagagagatt acaactccgg ctctggcgag
     1021 gcccagaaca actatccagc ctggaggcag agggagctcg ncaggatgag cccagaagaa
     1081 ctcctggcac tttcagcatc agactactac tgctggtgcc tggattacaa gggtgggcgc
     1141 gccgacccag ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt
     1201 tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc
     1261 ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct
     1321 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg
     1381 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg
     1441 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat
     1501 aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag
     1561 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga
     1621 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct
     1681 gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa
     1741 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg
     1801 cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag
     1861 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat
     1921 ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat
     1981 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta
     2041 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc
     2101 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat
     2161 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc
     2221 taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac
     2281 ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca
     2341 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     2401 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     2461 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     2521 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     2581 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     2641 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     2701 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     2761 cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     2821 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     2881 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     2941 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     3001 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     3061 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     3121 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     3181 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     3241 gcaattaata cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg
     3301 tcaggatggc cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc
     3361 accctccggg ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca
     3421 ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct
     3481 ttcgttttat ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt
     3541 tcccagtcac gacgttgtaa aacgacggcc agtcttaagc tcgggccc
//
