LOCUS       sequence-130515-         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Twister plasmid to swap inserts from an alpha2 or alpha2R vector to 
            any omega level vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pDGB1alpha1_SF (GB0106)
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    195..219
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     complement(390..409)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggtgtaacga attcgtctca ggagtctcaa tggtaacttt actctttatt taaccataca
       61 ttttttttta tttttttcac tttgttcttc atccactatt gttctttgtt catcttgaac
      121 aaaagctccc tccttctttg ttcttcatcc accattgttc ttcatcaatc atttcgctgt
      181 catgagacga attctgacag gatatattgg cgggtaaact aagtcgctgt atgtgtttgt
      241 ttgagatctc atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt
      301 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag
      361 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc
      421 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc
      481 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt
      541 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt
      601 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc
      661 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa
      721 gtggtggcct aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa
      781 gccagttacc ttcggaaga
//
