LOCUS       sequence-13053-e        3408 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3408)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3408)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1159-HQ858703
FEATURES             Location/Qualifiers
     source          1..3408
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(965..1064)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1081..1100)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1082..1100)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1105..1121)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1105..1121)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1223..1242)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1234..2043
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1301..1320)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2190..2778
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2679..2698
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2932..2949
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3108..3135)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3227..3313)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3362..3384
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3376..3393
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3377..3393
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atggcgacaa cgacggacct ggggttcgag gcgacggagc tccgcctggg cctgcccggc
      181 ggcagcggcg ggggaggggg aggggagctg gcgctgggcg gcgaggggag gagctcgtcc
      241 tccgcctccg gcaagagggg cttcgccgag accatcgacc tgaagctgaa gctggagcca
      301 gcggccgtcg tggaggcgga ggaggaggag gaggaccacg gcgttgctgt tgcccttgag
      361 aaggaggagg aggccgggaa gatgaagcgg tccccgagcc agagcagcgt cgccgccgcc
      421 gccgtgctgg ctgaccccgc cgagaagccg cgcgccgcca aggctcaggt ggtgggatgg
      481 ccaccggtcc ggtcgttccg gaagaacatc atgtccgtgc agtccgacaa gggcgccggc
      541 aaagacgccg ccgccgccaa cggcgacaag tcctcggcgg cggcaggcgg cggtgccgcg
      601 ttcgtgaagg tgagcttgga cggcgcgccc tacctgcgca aagtggacct caagatgtac
      661 aggagctacc agcagctgtc caaggcgctc gagaacatgt tcagctcctt caccatcgga
      721 agctgtgggt ctcaagggat gaacggcatg aacgagagca agctggtgga tctgctcaac
      781 ggctccgagt acgtgccgac ctacgaggac aaggacgggg actggatgct cgtcggcgat
      841 gtgccgtggg agatgttcgt cgaatcatgc aagcgccttc ggatcatgaa aggatcagaa
      901 gccattggcc tggcaccaag ggccatggag aaatgcaaga acagaagcaa gggtgggcgc
      961 gccgacccag ctttcttgta caaagttggc attataagaa agcattgctt atcaatttgt
     1021 tgcaacgaac aggtcactat cagtcaaaat aaaatcatta tttgccatcc agctgatatc
     1081 ccctatagtg agtcgtatta catggtcata gctgtttcct ggcagctctg gcccgtgtct
     1141 caaaatctct gatgttacat tgcacaagat aaaaatatat catcatgaac aataaaactg
     1201 tctgcttaca taaacagtaa tacaaggggt gttatgagcc atattcaacg ggaaacgtcg
     1261 aggccgcgat taaattccaa catggatgct gatttatatg ggtataaatg ggctcgcgat
     1321 aatgtcgggc aatcaggtgc gacaatctat cgcttgtatg ggaagcccga tgcgccagag
     1381 ttgtttctga aacatggcaa aggtagcgtt gccaatgatg ttacagatga gatggtcaga
     1441 ctaaactggc tgacggaatt tatgcctctt ccgaccatca agcattttat ccgtactcct
     1501 gatgatgcat ggttactcac cactgcgatc cccggaaaaa cagcattcca ggtattagaa
     1561 gaatatcctg attcaggtga aaatattgtt gatgcgctgg cagtgttcct gcgccggttg
     1621 cattcgattc ctgtttgtaa ttgtcctttt aacagcgatc gcgtatttcg tctcgctcag
     1681 gcgcaatcac gaatgaataa cggtttggtt gatgcgagtg attttgatga cgagcgtaat
     1741 ggctggcctg ttgaacaagt ctggaaagaa atgcataaac ttttgccatt ctcaccggat
     1801 tcagtcgtca ctcatggtga tttctcactt gataacctta tttttgacga ggggaaatta
     1861 ataggttgta ttgatgttgg acgagtcgga atcgcagacc gataccagga tcttgccatc
     1921 ctatggaact gcctcggtga gttttctcct tcattacaga aacggctttt tcaaaaatat
     1981 ggtattgata atcctgatat gaataaattg cagtttcatt tgatgctcga tgagtttttc
     2041 taatcagaat tggttaattg gttgtaacac tggcagagca ttacgctgac ttgacgggac
     2101 ggcgcaagct catgaccaaa atcccttaac gtgagttacg cgtcgttcca ctgagcgtca
     2161 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     2221 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     2281 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt
     2341 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     2401 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     2461 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     2521 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     2581 cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     2641 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     2701 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     2761 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     2821 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     2881 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     2941 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     3001 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     3061 gcaattaata cgcgtaccgc tagccaggaa gagtttgtag aaacgcaaaa aggccatccg
     3121 tcaggatggc cttctgctta gtttgatgcc tggcagttta tggcgggcgt cctgcccgcc
     3181 accctccggg ccgttgcttc acaacgttca aatccgctcc cggcggattt gtcctactca
     3241 ggagagcgtt caccgacaaa caacagataa aacgaaaggc ccagtcttcc gactgagcct
     3301 ttcgttttat ttgatgcctg gcagttccct actctcgcgt taacgctagc atggatgttt
     3361 tcccagtcac gacgttgtaa aacgacggcc agtcttaagc tcgggccc
//
