LOCUS       sequence-13055-e        3681 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3681)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3681)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1161-HQ858705
FEATURES             Location/Qualifiers
     source          1..3681
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1238..1337)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1354..1373)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1355..1373)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1378..1394)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1378..1394)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1496..1515)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1507..2316
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1574..1593)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2463..3051
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2952..2971
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3205..3222
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3381..3408)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3500..3586)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3635..3657
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3649..3666
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3650..3666
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atgacgcggc ggtgctcgca ctgcagcaac aacggccaca actcgcgtac ctgccccgtc
      181 cgctccgccg gcggcggcag cggcggcggc ggggtgaggc tgttcggcgt gcggctcacg
      241 acggcgccgg cgccggcggt gatgaagaag agcgccagca tgagctgcat tgtgtcctcg
      301 ctcggcggcg gtttcggcgg ctcgtcgccg ccggtggaag gagtgggtgc tggcagagga
      361 ggggaagaca gtgggaccgg ctacgtgtcc gatgatcccg cgcacgcatc ctgctcgacg
      421 aatggccgcg ccgagcgcaa gaaaggtaca ccttggactg aagaagagca tagaatgttt
      481 ctgatgggtc tgcagaagct tggtaaggga gactggcgcg ggatatccag aaactttgtt
      541 gtttccagga ccccgactca agttgcaagc catgctcaaa agtactttgt tagacagaca
      601 aactcatcga gacggaagag gcggtcaagc ttatttgaca tggtcccaga aatgccaatg
      661 gatgagtccc cagctgctgt ggaacaattt actctccaaa atactcaaga tgaagataca
      721 agttcaaatc acgtgccaac cttacatctt ggacaacgga aggaaacaga gtttgctaag
      781 cagctacaaa cttttcagcc agggcaccat gagaaatctg aatacgcaga accttcattg
      841 ctgctaccag atttggagat gaaccccagt gtaccattca agaccataac tgatccaaca
      901 ggaccagcat tctacccatc attggtccct gttccactaa ctctttggcc tccaagtgtt
      961 tcccacgtgg aggaagcagc cagaacccat gaaatcctaa aaccaactcc tttgaatggt
     1021 aagggggtgg ttaaggcaga tgatgttgtt ggtatgtcta agctcagcat cgacgaagct
     1081 agctctggct ccatggaacc cactgctctt tcccttgagc ttattggatc cacagataca
     1141 aggcaatcag cttttcatgt gagtccaccg atgaatagac ctgaactaag caagagaaac
     1201 agcagtccaa ttcatgcagt taagggtggg cgcgccgacc cagctttctt gtacaaagtt
     1261 ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa
     1321 aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc
     1381 atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa
     1441 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     1501 ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat
     1561 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
     1621 tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
     1681 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
     1741 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
     1801 atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
     1861 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
     1921 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
     1981 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
     2041 gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
     2101 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
     2161 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     2221 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     2281 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
     2341 cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt
     2401 aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2461 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2521 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2581 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     2641 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2701 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2761 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     2821 acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac gcttcccgaa
     2881 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     2941 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     3001 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3061 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3121 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3181 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     3241 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     3301 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag
     3361 gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat
     3421 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt
     3481 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
     3541 taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc
     3601 cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg
     3661 gccagtctta agctcgggcc c
//
