LOCUS       sequence-130551-         860 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pH-Sensitive Red Fluorescent Protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 860)
  AUTHORS   Fan Y, Chen Z, Ai HW
  TITLE     Monitoring redox dynamics in living cells with a redox-sensitive red
            fluorescent protein.
  JOURNAL   Anal Chem. 2015 Mar 3;87(5):2802-10. doi: 10.1021/ac5041988. Epub 
            2015 Feb 20.
  PUBMED    25666702
REFERENCE   2  (bases 1 to 860)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/ac5041988";
            journalName: "Anal Chem"; date: "2015-03-3- 3"; volume: "87"; issue:
            "5"; pages: "2802-10"
COMMENT     Sequence Label: pcDNA3-pHRFP
FEATURES             Location/Qualifiers
     source          1..860
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      85..94
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             103..120
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             124..156
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(160..168,169..183)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tgccgccacc atgggaggtt ctcatcatca tcatcatcat
      121 ggtatggcta gcatgactgg tggacagcaa atgggtcggg atctgtacga cgatgacgat
      181 aaggatccga gctcgagcgt tcatgctata ggtcggctga gctcacccgt ggtttccgag
      241 cagatgtacc ccgaggacgg cgccctgaag agcgagatca agaaggggct gaggctgaag
      301 gacggcggcc actacgccgc cgaggtcaag accacctaca aggccaagaa gcccgtgcag
      361 ctgcccggcg cctacatcgt cgacatcaag ttggacatcg tgtcccacaa cgaggactac
      421 accatcgtgg aacagtgcga acgcgccgag ggccgccact ccaccggcgg catggacgag
      481 ctgtacaagg gaggtacagg cgggagtctg gtgagcaagg gcgaggagga taacatggcc
      541 atcatcaagg agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag
      601 ttcgagatcg agggcgaggg cgagggccgc ccctacgagg cctttcagac cgctaagctg
      661 aaggtgacca agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg
      721 tacggctcca aggcctacat taagcaccca gccgacatcc ccgactactt caagctgtcc
      781 ttccccgagg gcttcaggtg ggagcgcgtg atgaacttcg aggacggcgg cattattcac
      841 gttaaccagg actcctccct
//
