LOCUS       sequence-130557-         529 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Egg cell-specific promoter-controlled expression of 
            3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target
            sequence (U6-26 promoter), Hyg resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 529)
  AUTHORS   Wang ZP, Xing HL, Dong L, Zhang HY, Han CY, Wang XC, Chen QJ
  TITLE     Egg cell-specific promoter-controlled CRISPR/Cas9 efficiently 
            generates homozygous mutants for multiple target genes in 
            Arabidopsis in a single generation.
  JOURNAL   Genome Biol. 2015 Jul 21;16:144. doi: 10.1186/s13059-015-0715-0.
  PUBMED    26193878
REFERENCE   2  (bases 1 to 529)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genome 
            Biol."; date: "2015-07-21"; volume: "16:144. doi"; pages: " 
            10.1186/s13059-015-0715-0"
COMMENT     Sequence Label: pHEE401E
FEATURES             Location/Qualifiers
     source          1..529
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 tcttcttagc tttttttctt cttcttcgtt catacagttt ttttttgttt atcagcttac
       61 attttcttga accgtagctt tcgttttctt ctttttaact ttccattcgg agtttttgta
      121 tcttgtttca tagtttgtcc caggattaga atgattaggc atcgaacctt caagaatttg
      181 attgaataaa acatcttcat tcttaagata tgaagataat cttcaaaagg cccctgggaa
      241 tctgaaagaa gagaagcagg cccatttata tgggaaagaa caatagtatt tcttatatag
      301 gcccatttaa gttgaaaaca atcttcaaaa gtcccacatc gcttagataa gaaaacgaag
      361 ctgagtttat atacagctag agtcgaagta gtgattggga gaccaaccca gtggacataa
      421 gcctgttcgg ttcgtaagct gtaatgcaag tagcgtatgc gctcacgcaa ctggtccaga
      481 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatgg
//
