LOCUS       sequence-13056-e        3621 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3621)
  AUTHORS   Burdo B, Gray J, Goetting-Minesky MP, Wittler B, Hunt M, Li T, 
            Velliquette D, Thomas J, Gentzel I, dos Santos Brito M, Mejia-Guerra
            MK, Connolly LN, Qaisi D, Li W, Casas MI, Doseff AI, Grotewold E
  TITLE     The Maize TFome--development of a transcription factor open reading 
            frame collection for functional genomics.
  JOURNAL   Plant J. 2014 Oct;80(2):356-66. doi: 10.1111/tpj.12623. Epub 2014 
            Aug 26.
  PUBMED    25053252
REFERENCE   2  (bases 1 to 3621)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1111/tpj.12623";
            journalName: "Plant J"; date: "2014-10"; volume: "80"; issue: "2"; 
            pages: "356-66"
COMMENT     Sequence Label: pUT1162-HQ858706
FEATURES             Location/Qualifiers
     source          1..3621
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     RBS             787..795
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     protein_bind    complement(1178..1277)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1294..1313)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1295..1313)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1318..1334)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1318..1334)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1436..1455)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             1447..2256
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(1514..1533)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      2403..2991
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2892..2911
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3145..3162
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3321..3348)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3440..3526)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     3575..3597
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3589..3606
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3590..3606
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccgcggccgc ccccttcacc
      121 atggcgtcgt cttctccgtc ctgccgtggc agcggcggca agtcggacgt ggaccggatc
      181 aagggcccct ggagccccga ggaggacgag gccctgcagc gcctggtggc ccgccacggc
      241 gcccgcaact ggtccctcat cagccggtcc atcccgggcc gctccggcaa gtcgtgccgc
      301 ctccggtggt gcaaccagct gtcgccgcag gtggagcacc ggcccttcac ggccgaggag
      361 gacgacacca tcctccgcgc gcacgccagg ttcggcaaca agtgggccac catcgcgcgc
      421 ctcctctccg gccgcaccga caacgccatc aagaaccact ggaactccac gctgaagcgc
      481 aagtactacg ccgccagcga cgctgttgtt gctgacgctg acgacgagcg cccgctgaag
      541 cgcacaagca gcgatggcca cccggggctc tgcttcagcc ccggaagccc atccgggtcc
      601 gacctcagcg actctagcaa ccacagcctt ccctccgtca tgccgtcagc cgcttctgct
      661 gccgccgtca cctcccacca gcagcagcag cacgtgtacc gccccgtgcc gcgcgcgggc
      721 ggcgtggtca tgctaccggt ggcgcctctt gtgccgccgc cgccgccgcc gccacagcct
      781 ccggcgacct ccttatcact gtccctctcc ctgcccgggc tggacggcca gaagcccgaa
      841 gcatccccag cggttctgac gccggcaccg gcaccgcctc aacctcctct tcagatacag
      901 cagcagcagc ctgctccacc tcagatgccg ccaccacaag cccaaccgag cttgccgttt
      961 cagctgcagg cagccgcgac aaactcacgt tcgcagcagc cggcagcagc gccgttcagt
     1021 ttggagttcc tgttgttgat gcaggagatg atccggatcg aggtacggaa ctacatgtcc
     1081 gggtcggggc tcgatccgcg caccgacggg gccgccgtgc acgccgtgag caagcgtatg
     1141 atgggtatgg ccaagatcga gaagggtggg cgcgccgacc cagctttctt gtacaaagtt
     1201 ggcattataa gaaagcattg cttatcaatt tgttgcaacg aacaggtcac tatcagtcaa
     1261 aataaaatca ttatttgcca tccagctgat atcccctata gtgagtcgta ttacatggtc
     1321 atagctgttt cctggcagct ctggcccgtg tctcaaaatc tctgatgtta cattgcacaa
     1381 gataaaaata tatcatcatg aacaataaaa ctgtctgctt acataaacag taatacaagg
     1441 ggtgttatga gccatattca acgggaaacg tcgaggccgc gattaaattc caacatggat
     1501 gctgatttat atgggtataa atgggctcgc gataatgtcg ggcaatcagg tgcgacaatc
     1561 tatcgcttgt atgggaagcc cgatgcgcca gagttgtttc tgaaacatgg caaaggtagc
     1621 gttgccaatg atgttacaga tgagatggtc agactaaact ggctgacgga atttatgcct
     1681 cttccgacca tcaagcattt tatccgtact cctgatgatg catggttact caccactgcg
     1741 atccccggaa aaacagcatt ccaggtatta gaagaatatc ctgattcagg tgaaaatatt
     1801 gttgatgcgc tggcagtgtt cctgcgccgg ttgcattcga ttcctgtttg taattgtcct
     1861 tttaacagcg atcgcgtatt tcgtctcgct caggcgcaat cacgaatgaa taacggtttg
     1921 gttgatgcga gtgattttga tgacgagcgt aatggctggc ctgttgaaca agtctggaaa
     1981 gaaatgcata aacttttgcc attctcaccg gattcagtcg tcactcatgg tgatttctca
     2041 cttgataacc ttatttttga cgaggggaaa ttaataggtt gtattgatgt tggacgagtc
     2101 ggaatcgcag accgatacca ggatcttgcc atcctatgga actgcctcgg tgagttttct
     2161 ccttcattac agaaacggct ttttcaaaaa tatggtattg ataatcctga tatgaataaa
     2221 ttgcagtttc atttgatgct cgatgagttt ttctaatcag aattggttaa ttggttgtaa
     2281 cactggcaga gcattacgct gacttgacgg gacggcgcaa gctcatgacc aaaatccctt
     2341 aacgtgagtt acgcgtcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     2401 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     2461 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     2521 ttcagcagag cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac
     2581 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     2641 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     2701 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     2761 acctacaccg aactgagata cctacagcgt gagcattgag aaagcgccac gcttcccgaa
     2821 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     2881 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     2941 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     3001 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     3061 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     3121 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     3181 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     3241 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atacgcgtac cgctagccag
     3301 gaagagtttg tagaaacgca aaaaggccat ccgtcaggat ggccttctgc ttagtttgat
     3361 gcctggcagt ttatggcggg cgtcctgccc gccaccctcc gggccgttgc ttcacaacgt
     3421 tcaaatccgc tcccggcgga tttgtcctac tcaggagagc gttcaccgac aaacaacaga
     3481 taaaacgaaa ggcccagtct tccgactgag cctttcgttt tatttgatgc ctggcagttc
     3541 cctactctcg cgttaacgct agcatggatg ttttcccagt cacgacgttg taaaacgacg
     3601 gccagtctta agctcgggcc c
//
