LOCUS       sequence-130567-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_871_Nhe2_EcR-to-ato
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    21..45
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(458..482)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      673..682
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(745..763)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
ORIGIN
        1 tttctctatc gataggtacc acaagtttgt acaaaaaagc aggcttcgtt ttcaaacagg
       61 tggtgtcaac actctgtaat caacaggtgg aaattaaaac accaaattga atttgtggaa
      121 ggcctaggcc actggttaga acaattgaat atggagacgg aaaaatgcat gaggcatcat
      181 cctaaattga ctaccaattt tatataatga gatgggcgac actcattcca gcagatagcg
      241 aataccaacg gatgcaattg ctcaagtcgg gacaattact gaatccgaca acaggtggag
      301 agatttatca gcatctcgaa aattacactc gatgtacaca ccaattgctc aattgggagt
      361 ctttagtcat tccgcactcg acagataagc tttcgctact gctgctaata aaatttcgcc
      421 aataatgcaa atcagatctt atcgcaggcc aactgggacc cagctttctt gtacaaagtg
      481 gtagatctgc gatcgagctc gcccggggat cgagcgcagc ggtataaaag ggcgcggggt
      541 ggctgagagc atcagttgtg aatgaatgtt cgagccgagc agacgtgccg ctgccttcgt
      601 taatatcctt tgaataagcc aactttgaat cacaagacgc ataccaaacg gcattccggt
      661 actgttggta aagccaccat ggaagacgcc aaaaacataa agaaaggccc ggcgccattc
      721 tatccgctgg aagatggaac cgctggagag caactgcata aggctatgaa gagatacgcc
      781 ctggttcctg gaacaattgc ttttacagat gcacatatcg aggtggacat cacttacgct
      841 gagtacttcg
//
