LOCUS       sequence-130569-         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Luciferase reporter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Stampfel G, Kazmar T, Frank O, Wienerroither S, Reiter F, Stark A
  TITLE     Transcriptional regulators form diverse groups with 
            context-dependent regulatory functions.
  JOURNAL   Nature. 2015 Nov 9. doi: 10.1038/nature15545.
  PUBMED    26550828
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature. 
            2015 Nov 9. doi: 10.1038/nature15545."
COMMENT     Sequence Label: pSGE_871_Nhe2_EcR-to-ato
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      36..45
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     protein_bind    236..260
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(673..697)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    711..802
                     /label=pause site
                     /note="RNA polymerase II transcriptional pause signal from 
                     the human alpha-2 globin gene"
     primer_bind     complement(743..762)
                     /label=RVprimer3
                     /note="pGL3 vector, forward primer"
ORIGIN
        1 tggcgccggg cctttcttta tgtttttggc gtcttccatg gtggctttac caacagtacc
       61 ggaatgccgt ttggtatgcg tcttgtgatt caaagttggc ttattcaaag gatattaacg
      121 aaggcagcgg cacgtctgct cggctcgaac attcattcac aactgatgct ctcagccacc
      181 ccgcgccctt ttataccgct gcgctcgatc cccgggcgag ctcgatcgca gatctaccac
      241 tttgtacaag aaagctgggt cccagttggc ctgcgataag atctgatttg cattattggc
      301 gaaattttat tagcagcagt agcgaaagct tatctgtcga gtgcggaatg actaaagact
      361 cccaattgag caattggtgt gtacatcgag tgtaattttc gagatgctga taaatctctc
      421 cacctgttgt cggattcagt aattgtcccg acttgagcaa ttgcatccgt tggtattcgc
      481 tatctgctgg aatgagtgtc gcccatctca ttatataaaa ttggtagtca atttaggatg
      541 atgcctcatg catttttccg tctccatatt caattgttct aaccagtggc ctaggccttc
      601 cacaaattca atttggtgtt ttaatttcca cctgttgatt acagagtgtt gacaccacct
      661 gtttgaaaac gaagcctgct tttttgtaca aacttgtggt acctatcgat agagaaatgt
      721 tctggcacct gcacttgcac tggggacagc ctattttgct agtttgtttt gtttcgtttt
      781 gttttgatgg agagcgtatg ttagtactat cgattcacac
//
