LOCUS       sequence-130584-         824 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway ORF clone of human RHOA [NM_001664.2] with stop codon (for 
            native or N-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 824)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 824)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E277 Hs.RHOA
FEATURES             Location/Qualifiers
     source          1..824
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    84..183
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(182..763)
                     /codon_start=1
                     /product="human Rho family small GTPase RhoA"
                     /label=RhoA
                     /translation="MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVAD
                     IEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVK
                     HFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKT
                     KDGVREVFEMATRAALQARRGKKKSGCLVL"
     protein_bind    complement(766..790)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gtgtgcgtca cccggcaacc ttgggtagca gcgaagtcga ggcatttctg tcctggctgg
       61 tctagaggat ctaagcttgc atgcaaataa tgattttatt ttgactgata gtgacctgtt
      121 cgttgcaaca aattgataag caatgctttc ctataatgcc aactttgtac aagaaagttg
      181 attacaagac aaggcaccca gattttttct tcccacgtct agcttgcaga gcagctctcg
      241 tagccatttc aaaaacctct ctcactccat ctttggtctt tgctgaacac tccatgtacc
      301 caaaagcgcc aatcctgttt gccatatctc tgccttcttc aggtttcacc ggctcctgct
      361 tcatcttggc tagctcccgc cttgtgtgct catcattccg aagatccttc ttattcccaa
      421 ccaggatgat gggcacgttg ggacagaaat gcttgacttc tggggtccac ttttctggga
      481 tgttttctaa actatcaggg ctgtcgatgg aaaaacacat cagtataaca tcggtatctg
      541 ggtaggagag gggcctcagg cgatcataat cttcctgccc agctgtgtcc cacaaagcca
      601 actctacctg ctttccatcc acctcgatat ctgccacata gttctcaaac actgtgggca
      661 catacacctc tgggaactgg tccttgctga agactatgag caagcatgtc tttccacagg
      721 ctccatcacc aacaatcacc agtttcttcc ggatggcagc catggtgcca acttttttgt
      781 acaaagttgg cattataaaa aagcattgct tatcaatttg ttgc
//
