LOCUS       sequence-130585-         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gateway ORF clone of human RHOA [NM_001664.2] without stop codon 
            (for C-terminal fusions).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  TITLE     Esposito lab
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: R777-E278 Hs.RHOA-nostop
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    86..185
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(184..762)
                     /codon_start=1
                     /product="human Rho family small GTPase RhoA"
                     /label=RhoA
                     /translation="MAAIRKKLVIVGDGACGKTCLLIVFSKDQFPEVYVPTVFENYVAD
                     IEVDGKQVELALWDTAGQEDYDRLRPLSYPDTDVILMCFSIDSPDSLENIPEKWTPEVK
                     HFCPNVPIILVGNKKDLRNDEHTRRELAKMKQEPVKPEEGRDMANRIGAFGYMECSAKT
                     KDGVREVFEMATRAALQARRGKKKSGCLVL"
     protein_bind    complement(765..789)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cggtgtgcgt cacccggcaa ccttgggtag cagcgaagtc gaggcatttc tgtcctggct
       61 ggtctagagg atctaagctt gcatgcaaat aatgatttta ttttgactga tagtgacctg
      121 ttcgttgcaa caaattgata agcaatgctt tcctataatg ccaactttgt acaagaaagt
      181 tgacaagaca aggcacccag attttttctt cccacgtcta gcttgcagag cagctctcgt
      241 agccatttca aaaacctctc tcactccatc tttggtcttt gctgaacact ccatgtaccc
      301 aaaagcgcca atcctgtttg ccatatctct gccttcttca ggtttcaccg gctcctgctt
      361 catcttggct agctcccgcc ttgtgtgctc atcattccga agatccttct tattcccaac
      421 caggatgatg ggcacgttgg gacagaaatg cttgacttct ggggtccact tttctgggat
      481 gttttctaaa ctatcagggc tgtcgatgga aaaacacatc agtataacat cggtatctgg
      541 gtaggagagg ggcctcaggc gatcataatc ttcctgccca gctgtgtccc acaaagccaa
      601 ctctacctgc tttccatcca cctcgatatc tgccacatag ttctcaaaca ctgtgggcac
      661 atacacctct gggaactggt ccttgctgaa gactatgagc aagcatgtct ttccacaggc
      721 tccatcacca acaatcacca gtttcttccg gatggcagcc atggtgccaa cttttttgta
      781 caaagttggc attataaaaa agcattgctt atcaatttgt tgcaacgaac aggtcactat
      841 cagtcaaaat aaaatcatta ttt
//
