LOCUS       sequence-130590-         716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses N-demethylases for the degredation of caffeine and related
            methylxanthines. Can be used to measure caffeine content as 
            described in the publication listed below..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Quandt EM, Hammerling MJ, Summers RM, Otoupal PB, Slater B, Alnahhas
            RN, Dasgupta A, Bachman JL, Subramanian MV, Barrick JE
  TITLE     Decaffeination and measurement of caffeine content by addicted 
            Escherichia coli with a refactored N-demethylation operon from 
            Pseudomonas putida CBB5.
  JOURNAL   ACS Synth Biol. 2013 Jun 21;2(6):301-7. doi: 10.1021/sb4000146. Epub
            2013 Mar 22.
  PUBMED    23654268
REFERENCE   2  (bases 1 to 716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/sb4000146";
            journalName: "ACS Synth Biol"; date: "2013-06-21- 21"; volume: "2"; 
            issue: "6"; pages: "301-7"
COMMENT     Sequence Label: pDCAF3-IS5
FEATURES             Location/Qualifiers
     source          1..716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    38..58
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
ORIGIN
        1 agggcagggt ggtgacacct tgcccttttt tgccggactg cagcggccgc tactagtact
       61 ctagtatcat tacttattga caggtttctt ccgcaagtga ggccggatcg aaggaagctg
      121 aggttggaaa aatccccgac atgacggtga atccggggat gcgtttgaca cggtccagcc
      181 agcgtcgtac cgccgggtag tcgagtagtg aaacgccggc ttcatccgac aatgcgatat
      241 acgggaagca ggcaagatcc gcgatggtgg gtgcggcggc cggacatagc cattgatgtc
      301 cattttgttc cgccgcccac aggtgttcgt ccagtatgcg cagtagttcg tgcgcgcggg
      361 cacggcattg ctctgcattg aaatcgtaaa aaaacaaatc atgcaaacgt gcagcggaaa
      421 tcgagccggt caaaccatcg gcaaacgcga gccacatatt tgcctggccg aggatggcgg
      481 gcgcatgtgt cggataccag gtcaaactgg tgtcgtattt gttcgccagg taaaccagga
      541 tggcctgggc atcgcgcaac acgaaatcat catcgcgcaa taccggcaac tggccgagtg
      601 gattgatgcg caggaaggcc ggtgatttat gttccctgga cgggaaaaat tccactgtct
      661 ctatcgtgta cggtagcttc aggatggaaa ggagcatccg caccttgtag caattc
//
