LOCUS       sequence-130597-         844 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces lentivirus expressing human FUS with FLAG tag at its 
            N-terminus by co-transfection with psPAX2 and pMD2G.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 844)
  AUTHORS   Nakaya T, Alexiou P, Maragkakis M, Chang A, Mourelatos Z
  TITLE     FUS regulates genes coding for RNA-binding proteins in neurons by 
            binding to their highly conserved introns.
  JOURNAL   RNA. 2013 Apr;19(4):498-509. doi: 10.1261/rna.037804.112. Epub 2013 
            Feb 6.
  PUBMED    23389473
REFERENCE   2  (bases 1 to 844)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1261/rna.037804.112"; journalName: "RNA"; date: "2013-04"; 
            volume: "19"; issue: "4"; pages: "498-509"
COMMENT     Sequence Label: pSLIK-NFLAG-hFUS
FEATURES             Location/Qualifiers
     source          1..844
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             58..81
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             462..473
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 ctccatagaa gacaccggga ccgatccagc ctccgcggcc ccgaactagt caccatggac
       61 tacaaggacg acgatgacaa aatggcctca aacgattata cccaacaagc aacccaaagc
      121 tatggggcct accccaccca gcccgggcag ggctattccc agcagagcag tcagccctac
      181 ggacagcaga gttacagtgg ttatagccag tccacggaca cttcaggcta tggccagagc
      241 agctattctt cttatggcca gagccagaac acaggctatg gaactcagtc aactccccag
      301 ggatatggct cgactggcgg ctatggcagt agccagagct cccaatcgtc ttacgggcag
      361 cagtcctcct atcctggcta tggccagcag ccagctccca gcagcacctc gggaagttac
      421 ggtagcagtt ctcagagcag cagctatggg cagccccaga gtgggagcta cagccagcag
      481 cctagctatg gtggacagca gcaaagctat ggacagcagc aaagctataa tccccctcag
      541 ggctatggac agcagaacca gtacaacagc agcagtggtg gtggaggtgg aggtggaggt
      601 ggaggtaact atggccaaga tcaatcctcc atgagtagtg gtggtggcag tggtggcggt
      661 tatggcaatc aagaccagag tggtggaggt ggcagcggtg gctatggaca gcaggaccgt
      721 ggaggccgcg gcaggggtgg cagtggtggc ggcggcggcg gcggcggtgg tggttacaac
      781 cgcagcagtg gtggctatga acccagaggt cgtggaggtg gccgtggagg cagaggtggc
      841 atgg
//
